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Effect of pH and dilution rate on specific production rate of extra cellular metabolites by Lactobacillus salivarius UCO_979C in continuous culture

  • Applied microbial and cell physiology
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Abstract

The effect of pH and dilution rate on the production of extracellular metabolites of Lactobacillus salivarius UCO_979 was studied. The experiments were carried out in continuous mode, with chemically defined culture medium at a temperature of 37 °C, 200 rpm agitation and synthetic air flow of 100 ml/min. Ethanol, acetic acid, formic acid, lactic acid and glucose were quantified through HPLC, while exopolysaccharide (EPS) was extracted with ethanol and quantified through the Dubois method. The results showed no linear trends for the specific production of lactic acid, EPS, acetic acid and ethanol, while the specific glucose consumption and ATP production rates showed linear trends. There was a metabolic change of the strain for dilution rates below 0.3 h−1. The pH had a significant effect on the metabolism of the strain, which was evidenced by a higher specific glucose consumption and increased production of ATP at pH 6 compared with that obtained at pH 7. This work shows not only the metabolic capabilities of L. salivarius UCO_979C, but also shows that it is possible to quantify some molecules associated with its current use as gastrointestinal probiotic, especially regarding the production of organic acids and EPS.

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Acknowledgements

This study received financial support from Conicyt, Chile (Javier Ferrer doctoral thesis) and Javier Ferrer Fondecyt thesis support (N° 24121273, Chile).

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Authors and Affiliations

  1. Department of Chemical Engineering, Universidad de Concepción, Campus Concepción, Concepción, Chile

    Javier Ferrer Valenzuela & Rodrigo Bórquez Yáñez

  2. Department of Microbiology, Universidad de Concepción, Campus Concepción, Concepción, Chile

    Luis Pinuer & Apolinaria García Cancino

  3. Department of Water Resources, Universidad de Concepción, Avda. Vicente Méndez 595, Chillán, Chile

    Javier Ferrer Valenzuela

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  1. Javier Ferrer Valenzuela
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  2. Luis Pinuer
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  3. Apolinaria García Cancino
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  4. Rodrigo Bórquez Yáñez
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Correspondence to Javier Ferrer Valenzuela.

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Javier Ferrer Valenzuela holds a doctorate degree, Universidad de Concepción.

Luis Pinuer holds an MD, Universidad de Concepción.

Dr. Apolinaria García Cancino holds a doctorate degree, Universidad de Concepción.

Dr. Rodrigo Bórquez Yáñez holds a doctorate degree, Universidad de Concepción.

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Valenzuela, J.F., Pinuer, L., Cancino, A.G. et al. Effect of pH and dilution rate on specific production rate of extra cellular metabolites by Lactobacillus salivarius UCO_979C in continuous culture. Appl Microbiol Biotechnol 99, 6417–6429 (2015). https://doi.org/10.1007/s00253-015-6526-0

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