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A novel integrated approach to quantitatively evaluate the efficiency of extracellular polymeric substances (EPS) extraction process

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Abstract

A novel integrated approach is developed to quantitatively evaluate the extracellular polymeric substances (EPS) extraction efficiency after taking into account EPS yield, EPS damage, and cell lysis. This approach incorporates grey relational analysis and fuzzy logic analysis, in which the evaluation procedure is established on the basis of grey relational coefficients generation, membership functions construction, and fuzzy rules description. The flocculation activity and DNA content of EPS are chosen as the two evaluation responses. To verify the feasibility and effectiveness of this integrated approach, EPS from Bacillus megaterium TF10 are extracted using five different extraction methods, and their extraction efficiencies are evaluated as one real case study. Based on the evaluation results, the maximal extraction grades and corresponding optimal extraction times of the five extraction methods are ordered as EDTA, 10 h > formaldehyde + NaOH, 60 min > heating, 120 min > ultrasonication, 30 min > H2SO4, 30 min > control. The proposed approach here offers an effective tool to select appropriate EPS extraction methods and determine the optimal extraction conditions.

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References

  • Burton K (1956) A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J 62:315–323

    CAS  Google Scholar 

  • Chiang KT, Chang FP, Tsai TC (2006) Optimum design parameters of Pin-Fin heat sink using the grey-fuzzy logic based on the orthogonal arrays. Int Commun Heat Mass 33:744–752

    Article  Google Scholar 

  • Comte S, Guibaud G, Baudu M (2006) Relation between extraction protocols for activated sludge extracellular polymeric substances (EPS) and EPS complexation properties Part I. Comparison of the efficiency of eight EPS extraction methods. Enzyme Microb Technol 38:237–245

    Article  CAS  Google Scholar 

  • D’Abzac P, Bordas F, van Hullebusch E, Lens PNL, Guibaud G (2010) Extraction of extracellular polymeric substances (EPS) from anaerobic granular sludges: comparison of chemical and physical extraction protocols. Appl Microbiol Biotechnol 85:1589–1599

    Article  Google Scholar 

  • Duru N, Dokmen F, Canbay MM, Kurtulus C (2010) Soil productivity analysis based on a fuzzy logic system. J Sci Food Agric 90:2220–2227

    Article  CAS  Google Scholar 

  • Fang F, Zeng RJ, Sheng GP, Yu HQ (2010) An integrated approach to identify the influential priority of the factors governing anaerobic H2 production by mixed cultures. Water Res 44:3234–3242

    Article  CAS  Google Scholar 

  • Frolund B, Griebe T, Nielsen PH (1996) Extraction of extracellular polymers from activated sludge using a cation exchange resin. Water Res 30:1749–1758

    Article  Google Scholar 

  • Jean MD, Lin BT, Chou JH (2006) Design of a fuzzy logic approach for optimization reinforced zirconia depositions using plasma sprayings. Surf Coat Technol 201:3129–3138

    Article  CAS  Google Scholar 

  • Klock JH, Wieland A, Seifert R, Michaelis W (2007) Extracellular polymeric substances (EPS) from cyanobacterial mats: characterisation and isolation method optimisation. Mar Biol 152:1077–1085

    Article  CAS  Google Scholar 

  • Lin JL, Lin CL (2005) The use of grey-fuzzy logic for the optimization of the manufacturing process. J Mater Process Tech 160:9–14

    Article  Google Scholar 

  • Liu H, Fang HHP (2002) Extraction of extracellular polymeric substances (EPS) of sludge. J Biotechnol 95:249–256

    Article  CAS  Google Scholar 

  • Liu NM, Horng JT, Chiang KT (2009) The method of grey-fuzzy logic for optimizing multi-response problems during the manufacturing process: a case study of the light guide plate printing process. Int J Adv Manuf Technol 41:200–210

    Article  Google Scholar 

  • Noorul HA, Marimuthu P, Jeyapaul R (2008) Multi response optimization of machining parameters of drilling Al/SiC metal matrix composite using grey relational analysis in the Taguchi method. Int J Adv Manuf Technol 37:250–255

    Article  Google Scholar 

  • Sheng GP, Yu HQ, Yu Z (2005) Extraction of extracellular polymeric substances from the photosynthetic bacterium Rhodopseudomonas acidophila. Appl Microbial Biotechnol 67:125–130

    Article  CAS  Google Scholar 

  • Sheng GP, Yu HQ, Li XX (2010) Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems: a review. Biotechnol Adv 28:882–894

    Article  CAS  Google Scholar 

  • Siddhi JH, Rajadurai A, Mohan B, Senthil KA, Sornakumar T (2009) Multi-response optimisation of sintering parameters of Al–Si alloy/fly ash composite using Taguchi method and grey relational analysis. Int J Adv Manuf Technol 45:362–369

    Article  Google Scholar 

  • Tepeli A, Hortacsu A (2008) A fuzzy logic approach for regulation in flux balance analysis. Biochem Eng J 39:137–148

    Article  CAS  Google Scholar 

  • Yuan SJ, Sun M, Sheng GP, Li Y, Li WW, Yao RS, Yu HQ (2011) Identification of key constituents and structure of the extracellular polymeric substances excreted by Bacillus megaterium TF10 for their flocculation capacity. Environ Sci Technol 45:1152–1157

    Article  CAS  Google Scholar 

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Acknowledgments

The authors wish to thank the NSFC (51129803), the NSFC-RGC Joint Project (21021140001), and the Fundamental Research Funds for the Central Universities (WK2060190007) for the partial support of this work.

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Authors and Affiliations

  1. Department of Chemistry, University of Science & Technology of China, Hefei, 230026, China

    Min Sun, Wen-Wei Li & Han-Qing Yu

  2. School of Chemical Engineering, Hefei University of Technology, Hefei, 230009, China

    Min Sun

  3. Department of Civil Engineering, Tohoku University, Sendai, 980-8579, Japan

    Hideki Harada

Authors
  1. Min Sun
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  2. Wen-Wei Li
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  3. Han-Qing Yu
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  4. Hideki Harada
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Correspondence to Han-Qing Yu.

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Sun, M., Li, WW., Yu, HQ. et al. A novel integrated approach to quantitatively evaluate the efficiency of extracellular polymeric substances (EPS) extraction process. Appl Microbiol Biotechnol 96, 1577–1585 (2012). https://doi.org/10.1007/s00253-012-4478-1

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  • DOI: https://doi.org/10.1007/s00253-012-4478-1

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