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A marine-derived Streptomyces sp. MS449 produces high yield of actinomycin X2 and actinomycin D with potent anti-tuberculosis activity

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Abstract

In the course of our screening program for anti-Mycobacterium bovis bacillus Calmette-Guérin (BCG) and anti-Mycobacterium tuberculosis H37Rv (MTB H37Rv) agents from our marine natural product library, a newly isolated actinomycete strain, designated as MS449, was picked out for further investigation. The strain MS449, isolated from a sediment sample collected from South China Sea, produced actinomycin X2 and actinomycin D in substantial quantities, which showed strong inhibition of BCG and MTB H37Rv. The structures of actinomycins were elucidated by nuclear magnetic resonance and mass spectrometric analysis. The strain MS449 was taxonomically characterized on the basis of morphological and phenotypic characteristics, genotypic data, and phylogenetic analysis. The 16S rRNA gene sequence of the strain was determined and a database search indicated that the strain was closely associated with the type strain of Streptomyces avermitilis (99.7 % 16S rRNA gene similarity). S. avermitilis has not been previously reported to produce actinomycins. The marine-derived strain of Streptomyces sp. MS449 produced notably higher quantities of actinomycin X2 (1.92 mg/ml) and actinomycin D (1.77 mg/ml) than previously reported actinomycins producing strains. Thus, MS449 was considered of great potential as a new industrial producing strain of actinomycin X2 and actinomycin D.

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Acknowledgments

This research was partly supported by the National Natural Science Foundation of China (30973665, 30911120483, 30911120484, 81102356, and 81102369), the CAS Pillar Program (XDA04074000), the Ministry of Science and Technology of China (2007DFB31620, 2011ZX09102-011-11, 2012CB721006), and China Postdoctoral Science Foundation (49th). L. Z. is an Awardee for National Distinguished Young Scholar Program in China.

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Authors and Affiliations

  1. Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100190, China

    Caixia Chen, Fuhang Song, Qian Wang, Wael M. Abdel-Mageed, Hui Guo, Chengzhang Fu, Huanqin Dai, Xueting Liu, Na Yang, Feng Xie, Ke Yu & Lixin Zhang

  2. Pharmacognosy Department, Faculty of Pharmacy, Assiut University, Assiut, Egypt

    Wael M. Abdel-Mageed

  3. Laboratory of Molecular Vector Biology, Haidian Center for Disease Prevention and Control, Haidian District, Beijing, 100094, People’s Republic of China

    Weiyuan Hou

  4. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100051, China

    Ruxian Chen

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  1. Caixia Chen
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  2. Fuhang Song
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Correspondence to Ruxian Chen or Lixin Zhang.

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Caixia Chen, Fuhang Song, and Qian Wang contributed equally to this paper.

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Chen, C., Song, F., Wang, Q. et al. A marine-derived Streptomyces sp. MS449 produces high yield of actinomycin X2 and actinomycin D with potent anti-tuberculosis activity. Appl Microbiol Biotechnol 95, 919–927 (2012). https://doi.org/10.1007/s00253-012-4079-z

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  • DOI: https://doi.org/10.1007/s00253-012-4079-z

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