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Effect of pretreatment of hydrothermally processed rice straw with laccase-displaying yeast on ethanol fermentation

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Abstract

A gene encoding laccase I was identified and cloned from the white-rot fungus Trametes sp. Ha1. Laccase I contained 10 introns and an original secretion signal sequence. After laccase I without introns was prepared by overlapping polymerase chain reaction, it was inserted into expression vector pULD1 for yeast cell surface display. The oxidation activity of a laccase-I-displaying yeast as a whole-cell biocatalyst was examined with 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), and the constructed yeast showed a high oxidation activity. After the pretreatment of hydrothermally processed rice straw (HPRS) with laccase-I-displaying yeast with ABTS, fermentation was conducted with yeast codisplaying endoglucanase, cellobiohydrolase, and β-glucosidase with HPRS. Fermentation of HPRS treated with laccase-I-displaying yeast was performed with 1.21-fold higher activities than those of HPRS treated with control yeast. The results indicated that pretreatment with laccase-I-displaying yeast with ABTS was effective for direct fermentation of cellulosic materials by yeast codisplaying endoglucanase, cellobiohydrolase, and β-glucosidase.

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Acknowledgment

This work was supported by a research fellowship from the Japan Society for the Promotion of Science for Young Scientists.

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Authors and Affiliations

  1. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan

    Akihito Nakanishi, Jun Gu Bae, Kotaro Fukai, Naoki Tokumoto, Kouichi Kuroda, Jun Ogawa, Sakayu Shimizu & Mitsuyoshi Ueda

  2. Daiwa Kasei, Konan, Shiga, 520-3203, Japan

    Masato Nakatani

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  1. Akihito Nakanishi
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  2. Jun Gu Bae
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  3. Kotaro Fukai
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  4. Naoki Tokumoto
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  5. Kouichi Kuroda
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  6. Jun Ogawa
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  7. Masato Nakatani
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  8. Sakayu Shimizu
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  9. Mitsuyoshi Ueda
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Correspondence to Mitsuyoshi Ueda.

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Nakanishi, A., Bae, J.G., Fukai, K. et al. Effect of pretreatment of hydrothermally processed rice straw with laccase-displaying yeast on ethanol fermentation. Appl Microbiol Biotechnol 94, 939–948 (2012). https://doi.org/10.1007/s00253-012-3876-8

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  • DOI: https://doi.org/10.1007/s00253-012-3876-8

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