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Transcriptome profiling of degU expression reveals unexpected regulatory patterns in Bacillus megaterium and discloses new targets for optimizing expression

  • Genomics, Transcriptomics, Proteomics
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An Erratum to this article was published on 10 November 2011

Abstract

The first whole transcriptome assessment of a Bacillus megaterium strain provides unanticipated insights into the degSU regulon considered to be of central importance for exo-enzyme production. Regulatory patterns as well as the transcription of degSU itself deviate from the model organism Bacillus subtilis; the number of DegU-regulated secretory enzymes is rather small. Targets for productivity optimization, besides degSU itself, arise from the unexpected DegU-dependent induction of the transition-state regulator AbrB during exponential growth. Induction of secretion-assisting factors, such as the translocase subunit SecY or the signal peptidase SipM, promote hypersecretion. B. megaterium DegSU transcriptional control is advantageous for production purposes, since the degU32 constitutively active mutant conferred hypersecretion of a heterologous Bacillus amyloliquefaciens amylase without the detrimental rise, as for B. subtilis and Bacillus licheniformis, in extracellular proteolytic activities.

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Acknowledgements

This work was supported by the Federal Ministry of Education and Research (BMBF), grant no. 0315283. This work was financially supported by “Deutsche Forschungsgemeinschaft (SFB578)”.

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  1. Kristina Hoffmann

    Present address: Forschungszentrum Jülich, Institut für Bio- und Geowissenschaften, Jülich, Germany

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  1. Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms Universität, Corrensstrasse 3, 48149, Münster, Germany

    Claudia Borgmeier, Kristina Hoffmann & Friedhelm Meinhardt

  2. Institut für Mikrobiologie, Technische Universität Braunschweig, Braunschweig, 38106, Niedersachsen, Germany

    Rebekka Biedendieck & Dieter Jahn

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An erratum to this article can be found at http://dx.doi.org/10.1007/s00253-011-3655-y

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Borgmeier, C., Biedendieck, R., Hoffmann, K. et al. Transcriptome profiling of degU expression reveals unexpected regulatory patterns in Bacillus megaterium and discloses new targets for optimizing expression. Appl Microbiol Biotechnol 92, 583–596 (2011). https://doi.org/10.1007/s00253-011-3575-x

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