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Isolation of basidiomycetous yeast Pseudozyma tsukubaensis and production of glycolipid biosurfactant, a diastereomer type of mannosylerythritol lipid-B

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Abstract

The producers of glycolipid biosurfactant, mannosylerythritol lipid-B (MEL-B), were isolated from leaves of Perilla frutescens on Ibaraki in Japan. Four isolates, 1D9, 1D10, 1D11, and 1E5, were identified as basidiomycetous yeast Pseudozyma tsukubaensis by rDNA sequence and biochemical properties. The structure of MEL-B produced by these strains was analyzed by 1H nuclear magnetic resonance and gas chromatography–mass spectrometry methods, and was determined to be the same as the diastereomer MEL-B produced by P. tsukubaensis NBRC 1940. Of these isolates, P. tsukubaensis 1E5 (JCM 16987) is capable of producing the largest amount of the diastereomer MEL-B from vegetable oils. In order to progress the diastereomer MEL-B production by strain 1E5, factors affecting the production, such as carbon and organic nutrient sources, were further examined. Olive oil and yeast extract were the best carbon and nutrient sources, respectively. Under the optimal conditions, a maximum yield, productivity, and yield coefficient of 73.1 g/L, 10.4 g L−1 day−1, and 43.5 g/g were achieved by feeding of olive oil in a 5-L jar-fermenter culture using strain 1E5.

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Acknowledgments

The authors thank Mr. Shun Azami and Dr. Shunichi Miyakoshi for their technical assistance. This study was supported by the Industrial Technology Research Grant Program in 06A17501c from the New Energy and Industrial Technology Development Organization (NEDO) of Japan.

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Authors and Affiliations

  1. Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, Higashi 1-1, Tsukuba, Ibaraki, 305-8565, Japan

    Tomotake Morita, Tokuma Fukuoka, Masaaki Konishi, Tomohiro Imura & Dai Kitamoto

  2. Japan Collection of Microorganisms, RIKEN BioResource Center, Hirosawa 2-1, Wako, Saitama, 351-0198, Japan

    Masako Takashima

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  1. Tomotake Morita
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  2. Masako Takashima
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  3. Tokuma Fukuoka
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  5. Tomohiro Imura
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  6. Dai Kitamoto
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Correspondence to Dai Kitamoto.

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Morita, T., Takashima, M., Fukuoka, T. et al. Isolation of basidiomycetous yeast Pseudozyma tsukubaensis and production of glycolipid biosurfactant, a diastereomer type of mannosylerythritol lipid-B. Appl Microbiol Biotechnol 88, 679–688 (2010). https://doi.org/10.1007/s00253-010-2762-5

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  • DOI: https://doi.org/10.1007/s00253-010-2762-5

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