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Formation and analysis of mannosylerythritol lipids secreted by Pseudozyma aphidis

  • Applied Microbial and Cell Physiology
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Abstract

Pseudozyma aphidis DSM 70725 was found to be a novel producer of mannosylerythritol lipids (MELs). The MELs were quantified by HPLC. Glucose as carbon source for precultivation supported growth well. By contrast, at concentrations >30 g l−1 in preculture, subsequent MEL formation in the main culture with soybean oil as sole carbon source was reduced. The type of substrate supply considerably influenced MEL formation. High concentrations of soybean oil (80 ml l−1) at init favored the production process when compared to a stepwise (20 ml l−1) addition. Mannose or erythritol were suitable second carbon sources that enhanced the MEL yield with soybean oil as preferred primary substrate. After 10 days, a maximum yield of 75 g l−1 was attained during shake-flask cultivation. Biofuel (rapeseed oil methyl ester) also resulted in high yields of MEL, but glucose reduced the MEL yield. Analysis by GC-MS showed that all fatty acids contained in MEL and derived from soybean oil or related methyl ester were degraded by C2-units to differing extents. The surface (water/air) and interfacial (water/hexadecane) tension of the MELs produced from different carbon sources were reduced to a minimum of 26.2 mN m−1 and 1 mN m−1, respectively.

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Acknowledgements

We thank W. Grassl and A. Walzog for technical assistance.

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Authors and Affiliations

  1. Institute of Biochemistry and Biotechnology, Technical University Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany

    U. Rau & S. Lang

  2. C12 P4 Khu tap the Kim Giang, Hoang Dao Thanh str., Ngo 102, Thanh xuan District, Hanoi, Vietnam

    L. A. Nguyen

  3. Institute of Organic Chemistry, Technical University Braunschweig, Hagenring 30, 38106, Braunschweig, Germany

    S. Schulz

  4. Department of Structural Biology, German Research Centre for Biotechnology, Mascherorder Weg 1, 38124, Braunschweig, Germany

    V. Wray & M. Nimtz

  5. Cargill R&D Europe, Havenstraat 84, 1800, Vilvoorde, Belgium

    H. Roeper & H. Koch

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  1. U. Rau
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  2. L. A. Nguyen
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  3. S. Schulz
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  4. V. Wray
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  8. S. Lang
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Correspondence to U. Rau.

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Rau, U., Nguyen, L.A., Schulz, S. et al. Formation and analysis of mannosylerythritol lipids secreted by Pseudozyma aphidis. Appl Microbiol Biotechnol 66, 551–559 (2005). https://doi.org/10.1007/s00253-004-1672-9

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  • DOI: https://doi.org/10.1007/s00253-004-1672-9

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