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Characterization of an adenylate cyclase gene (cyaB) deletion mutant of Corynebacterium glutamicum ATCC 13032

  • Applied Genetics and Molecular Biotechnology
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An Erratum to this article was published on 02 February 2010

Abstract

Genome analysis of C. glutamicum ATCC 13032 has showed one putative adenylate cyclase gene, cyaB (cg0375) which encodes membrane protein belonging to class III adenylate cyclases. To characterize the function of cyaB, a deletion mutant was constructed, and the mutant showed decreased level of intracellular cyclic AMP compared to that of wild-type. Interestingly, the cyaB mutant displayed growth defect on acetate medium, and this effect was reversed by complementation with cyaB gene. Similarly, it showed growth defect on glucose-acetate mixture minimal medium, and the utilization of glucose was retarded in the presence of acetate. The deletion mutant retained the activity of glyoxylate bypass enzymes. Additionally, the mutant could grow on ethanol but not on propionate medium. The data obtained from this study suggests that adenylate cyclase plays an essential role in the acetate metabolism of C. glutamicum, even though detailed regulatory mechanisms involving cAMP are not yet clearly defined. The observation that glyoxylate bypass enzymes are derepressed in cyaB mutant indicates the involvement of cAMP in the repression of aceB and aceA.

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Acknowledgements

This work was supported by the 21C Frontier Program of Microbial Genomics and Applications.

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Authors and Affiliations

  1. Department of Life Science & Genetic Engineering, Paichai University, Seo-Gu, Daejeon, 302-735, Korea

    Bindu Subhadra & Jung-Kee Lee

  2. Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 305-806, Korea

    Pu Hyeon Cha, Sun-Yung Park, Min-Woo Moon, Tae-Kwang Oh & Jihyun F. Kim

  3. Department of Biology, Yonsei University, Seoul, 120-749, Korea

    Eungbin Kim

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  1. Pu Hyeon Cha
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  2. Sun-Yung Park
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  3. Min-Woo Moon
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  7. Jihyun F. Kim
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Correspondence to Jihyun F. Kim or Jung-Kee Lee.

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Pu Hyeon Cha and Sun-Yung Park equally contributed to this work.

An erratum to this article can be found at http://dx.doi.org/10.1007/s00253-010-2436-3

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Cha, P.H., Park, SY., Moon, MW. et al. Characterization of an adenylate cyclase gene (cyaB) deletion mutant of Corynebacterium glutamicum ATCC 13032. Appl Microbiol Biotechnol 85, 1061–1068 (2010). https://doi.org/10.1007/s00253-009-2066-9

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  • DOI: https://doi.org/10.1007/s00253-009-2066-9

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