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Carrier-mediated acetate uptake in Corynebacterium glutamicum

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Abstract

Acetate is effectively taken up by whole cells of Corynebacterium glutamicum via a specific carrier with a pH optimum of 8. The K m of acetate uptake was 50 μM and the V max 25–35 nmol/mg dw min. The activation energy was determined to be 70 kJ/mol. Acetate uptake was competitively inhibited by propionate with a K i of about 30 μM and blocked by addition of sulfhydryl reagents. The transport activity was clearly dependent on the membrane potential, but independent of the presence of Na+-ions. It is concluded that uptake of acetate proceeds by a secondary, proton coupled mechanism.

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Abbreviations

CCCP:

carbonyloyanide-m-(chlorophenyl)-hydrazone

dw:

dry weight

PCMB:

p-(chloromercuri)benzoate

PCMS:

p-(chloromercuri)benzenesulfonate

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Authors and Affiliations

  1. Institut für Biotechnologie, Forschungszentrum Jülich, Postfach 1913, W-5170, Jülich, Germany

    Holger Ebbighausen, Brita Weil & Reinhard Krämer

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  1. Holger Ebbighausen
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  2. Brita Weil
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  3. Reinhard Krämer
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Ebbighausen, H., Weil, B. & Krämer, R. Carrier-mediated acetate uptake in Corynebacterium glutamicum . Arch. Microbiol. 155, 505–510 (1991). https://doi.org/10.1007/BF00244970

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  • DOI: https://doi.org/10.1007/BF00244970

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