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Involvement of nitric oxide in cerebroside-induced defense responses and taxol production in Taxus yunnanensis suspension cells

  • Applied Microbial and Cell Physiology
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Abstract

This work was to characterize the generation of nitric oxide (NO) in Taxus yunnanensis cells induced by a fungal-derived cerebroside and the signal role of NO in the elicitation of plant defense responses and taxol production. (2S,2′R,3R,3′E,4E,8E)-1-O-β-d-glucopyranosyl-2-N-(2′-hydroxy-3′-octadecenoyl)-3-hydroxy-9-methyl-4,8-sphingadienine at 10 μg/ml induced a rapid and dose-dependent NO production in the Taxus cell culture, reaching a maximum within 5 h of the treatment. The NO donor sodium nitroprusside (SNP) potentiated cerebroside-induced H2O2 production and cell death. Inhibition of nitric oxide synthase activity by phenylene-1,3-bis(ethane-2-isothiourea) dihydrobromide or scavenging NO by 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide partially blocked the cerebroside-induced H2O2 production and cell death. Moreover, NO enhanced cerebroside-induced activation of phenylalanine ammonium-lyase and accumulation of taxol in cell cultures. These results are suggestive of a role for NO as a new signal component for activating the cerebroside-induced defense responses and secondary metabolism activities of plant cells.

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Acknowledgments

This work was supported by grants for R.X.T. from NSFC (30470191) and for J.W.W. from NSF for universities in Jiangsu Province (05KJB360120), MSDF of Soochow University (EE132514).

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Authors and Affiliations

  1. School of Pharmaceutical Sciences, Soochow University, Suzhou, 215123, People’s Republic of China

    Jian Wen Wang

  2. Department of Horticultural Sciences, Soochow University, Suzhou, 215123, People’s Republic of China

    Li Ping Zheng

  3. Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, 210093, People’s Republic of China

    Ren Xiang Tan

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  1. Jian Wen Wang
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  2. Li Ping Zheng
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Correspondence to Ren Xiang Tan.

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Taxol is a trademark of Bristol-Myers Squibb, Madison, NJ.

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Wang, J.W., Zheng, L.P. & Tan, R.X. Involvement of nitric oxide in cerebroside-induced defense responses and taxol production in Taxus yunnanensis suspension cells. Appl Microbiol Biotechnol 75, 1183–1190 (2007). https://doi.org/10.1007/s00253-007-0927-7

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  • DOI: https://doi.org/10.1007/s00253-007-0927-7

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