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Nitric oxide mediates hypocrellin accumulation induced by fungal elicitor in submerged cultures of Shiraia bambusicola

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Abstract

Multiple responses of Shiraia bambusicola, including nitric oxide (NO) generation, hypocrellins production and salicylic acid (SA) biosynthesis, were induced by a fungal elicitor prepared from the mycelium of Aspergillum niger. Both the NO donator, sodium nitroprusside, and SA enhanced hypocrellin production without the fungal elicitor. However, the NO scavenger, 2,4-carboxyphenyl-4,4, 5,5- tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) and the SA biosynthesis inhibitor, cinnamic acid (CA), inhibited hypocrellin accumulation in the presence of the elicitor. cPTIO also inhibited SA production induced by the A. niger elicitor. CA failed to inhibit NO production but it significantly inhibited hypocrellin accumulation. Aspergillum niger elicitor induced an NO burst, SA accumulation, and hypocrellin production in S. bambusicola. Therefore, the fungal elicitor was involved in the signaling pathway, which is a mechanism different from that of higher plants.

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References

  • Bellin D, Asai S, Delledonne M, Yoshioka H (2013) Nitric oxide as a mediator for defense responses. Mol Plant Microb Interact 26:271–277

    Article  CAS  Google Scholar 

  • Du W, Sun C, Liang Z, Han Y, Yu J (2012) Antibacterial activity of hypocrellin A against Staphylococcus aureus. World J Microbiol Biotechnol 28:3151–3157

    Article  CAS  PubMed  Google Scholar 

  • Du W, Liang Z, Zou X, Han Y, Liang J, Yu J, Chen W, Wang Y, Sun C (2013) Effects of microbial elicitor on production of hypocrellin by Shiraia bambusicola. Folia Microbiol 58:283–289

    Article  CAS  Google Scholar 

  • Gao FK, Ren CG, Dai CC (2012) Signaling effects of nitric oxide, salicylic acid, and reactive oxygen species on isoeuphpekinensin accumulation in Euphorbia pekinensis suspension cells induced by an endophytic fungal elicitor. J Plant Growth Regul 31:490–497

    Article  CAS  Google Scholar 

  • Liang ZS, Yang DF, Liang X, Zhang YJ, Liu Y, Li FH (2012) Roles of reactive oxygen species in methyl jasmonate and nitric oxide-induced tanshinone production in Salvia miltiorrhiza hairy roots. Plant Cell Rep 31:873–883

    Article  CAS  PubMed  Google Scholar 

  • Modafar CE, Tantaoui A, Boustani ESE (2001) Differential induction of phenylalanine ammonia-lyase activity in date palm roots in response to inoculation with Fusarium oxysporum f. sp. albedinis and to elicitation with fungal wall elicitor. Plant Physiol 158:715–722

    Article  Google Scholar 

  • Monjil MS, Shibata Y, Takemoto D, Kawakita K (2013) Bis-aryl methanone compound is a candidate of nitric oxide producing elicitor and induces resistance in Nicotiana benthamiana against Phytophthora infestans. Nitric Oxide 29:34–45

    Article  CAS  PubMed  Google Scholar 

  • Mori T, Sakurai M, Sakuta M (2001) Effects of conditioned medium on activities of PAL, CHS, DAHP synthase (DS-Co and DS-Mn) and anthocyanin production in suspension cultures of Fragaria ananassa. Plant Sci 160:355–360

    Article  CAS  PubMed  Google Scholar 

  • Nurnberger T, Colling C, Hahlbrock K, Hahlbrock K, Jabs T, Renelt A, Sacks WR, Scheel D (1994) Perception and transduction of an elicitor signal in cultured parsley cells. Biochem Soc Symp 60:173–182

    CAS  PubMed  Google Scholar 

  • Roberts SC, Shuler ML (1997) Large-scale plant cell culture. Curr Opin Biotechnol 8:154–159

    Article  CAS  PubMed  Google Scholar 

  • Romero-Puertas MC, Perazzolli M, Zago ED, Delledonne M (2004) Nitric oxide signaling functions in plant-pathogen interactions. Cell Microbiol 6:795–803

    Article  CAS  PubMed  Google Scholar 

  • Wang Y, Dai CC, Zhao YW, Peng Y (2011) Fungal endophyte-induced volatile oil accumulation in Atractylodes lancea plantlets is mediated by nitric oxide, salicylic acid and hydrogen peroxide. Proc Biochem 46:730–735

    Article  Google Scholar 

  • Xu MJ, Dong JF, Zhu MY (2005) Nitric oxide mediates the fungal elicitor-induced hypericin production of Hypericum perforatum cell suspension cultures through a jasmonic-acid-dependent signal pathway. Plant Physiol 139:991–998

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Zhang B, Zou T, Lu YH, Wang JW (2010) Stimulation of artemisinin biosynthesis in Artemisia annua hairy roots by oligogalacturonides. Afr J Biotechnol 9:3437–3442

    CAS  Google Scholar 

  • Zhang M, Dong JF, Jin HH, Sun LN, Xu MJ (2011) Ultraviolet-B-induced flavonoid accumulation in Betula pendula leaves is dependent upon nitrate reductase-mediated nitric oxide signaling. Tree Physiol 31:798–807

    Article  CAS  PubMed  Google Scholar 

  • Zheng W, Zhao Y, Zheng X, Liu Y, Pan S, Dai Y, Liu F (2011) Production of antioxidant and antitumor metabolites by submerged cultures of Inonotus obliquus cocultured with Phellinus punctatus. Appl Microbiol Biotechnol 89:157–167

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (No. 30960004); Science Technology Foundation of Guizhou Province (No. 2014-2039); 2014 Doctor Foundation of Guiyang College of Traditional Chinese Medicine.

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Authors and Affiliations

  1. School of Basic Medical Science, Guiyang College of Traditional Chinese Medicine, Guiyang, China

    Wen Du & Jiandong Liang

  2. Institute of Fungus Resources, Guizhou University, Guiyang, China

    Yanfeng Han & Zongqi Liang

  3. Institute of Biochemistry and Nutrition, Guizhou University, Guiyang, China

    Jianping Yu

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  1. Wen Du
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  2. Jiandong Liang
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  3. Yanfeng Han
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  4. Jianping Yu
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  5. Zongqi Liang
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Correspondence to Wen Du.

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Du, W., Liang, J., Han, Y. et al. Nitric oxide mediates hypocrellin accumulation induced by fungal elicitor in submerged cultures of Shiraia bambusicola . Biotechnol Lett 37, 153–159 (2015). https://doi.org/10.1007/s10529-014-1665-4

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  • DOI: https://doi.org/10.1007/s10529-014-1665-4

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