Abstract
First, the effect of different levels of nitrogen source on clavulanic acid (CA) production was evaluated in batch cultivations utilizing complex culture medium containing glycerol and three different levels of soy protein isolate (SPI). Cellular growth, evaluated in terms of the rheological parameter K, was highest with a SPI concentration of 30 g.L−1 (4.42 g.L−1 N total). However, the highest production of CA (380 mg.L−1) was obtained when an intermediate concentration of 20 g.L−1 of SPI (2.95 g.L−1 total N) was used. To address this, the influences of volumetric flow rate (F) and glycerol concentration in the complex feed medium (CsF) in fed-batch cultivations were investigated. The best experimental condition for CA production was F=0.01 L.h−1 and CsF=120 g.L−1, and under these conditions maximum CA production was practically twice that obtained in the batch cultivation. A single empirical equation was proposed to relate maximum CA production with F and CsF in fed-batch experiments.
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Acknowledgements
The authors gratefully acknowledge Bunge Alimentos S/A for providing the samples of Samprosoy 90NB and the financial support from Fundação de Amparo à Pesquisa do Estado de São Paulo (Grant Proc. 03/11722-5 and PD Scholarship Proc.04/00224-7), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, and Conselho Nacional de Desenvolvimento Científico e Tecnológico.
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Teodoro, J.C., Baptista-Neto, A., Cruz-Hernández, I.L. et al. Influence of feeding conditions on clavulanic acid production in fed-batch cultivation with medium containing glycerol. Appl Microbiol Biotechnol 72, 450–455 (2006). https://doi.org/10.1007/s00253-005-0273-6
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DOI: https://doi.org/10.1007/s00253-005-0273-6