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Reference strand conformational analysis (RSCA) is a valuable tool in identifying MHC-DRB sequences in three species of Aotus monkeys

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Abstract

The Aotus monkey has been of great value in the pre-clinical study of malaria vaccine candidates. Several components of this primate’s immune system have been studied and they display great similarity to their human counterparts. Cloning and sequencing studies have revealed extensive sequence polymorphisms in Aotus MHC-DRB with very high similarities to several human allelic lineages, grouping at least nine distinct MHC-DRB lineages. As the efficacy of peptide vaccines in this animal model may be strongly influenced by exon 2 MHC-DRB polymorphism, the availability of a reliable and rapid MHC-DRB typing method for three species of Aotus (Aotus nancymaae, Aotus vociferans and Aotus nigriceps) is necessary. Reference strand conformational analysis (RSCA) was used here for differentiating the distinctive Aotus MHC-DRB sequences’ mobility using five fluorescently labelled references proved to be very useful for resolving closely related sequences, establishing the number of sequences transcribed in a particular monkey and their identity. The RSCA method’s reliability in terms of identifying Aotus MHC-DRB sequences will facilitate evaluating individual responsiveness to vaccines and prompt studies associating susceptibility/resistance to infectious agents or auto-immune disease, for which Aotus monkeys may be considered to be an appropriate animal model.

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Acknowledgements

We would like to thank Dr. Ciro Casadiego, Sandra Ramirez and Lida Triviño from the Universidad del Rosario’s Cellular and Molecular Biology Laboratory. This research has been supported by the Colombian President’s office and the Colombian Ministry of Health. The experiments reported here comply with current Colombian Ministry of Health regulations. We would especially like to thank Jason Garry for patiently reading this manuscript and correcting it.

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Authors and Affiliations

  1. Fundación Instituto de Inmunologia de Colombia (FIDIC), Carrera 50 Número 26-00, Bogotá, Colombia, South America

    Juan E. Baquero, Santiago Miranda, Oscar Murillo, Esperanza Trujillo, Carlos Suarez, Manuel E. Patarroyo & Carlos Parra-López

  2. Facultad de Ciencias, Universidad Nacional de Colombia, Bogotá, Colombia, South America

    Manuel E. Patarroyo & Carlos Parra-López

  3. Laboratorio de Biología Celular y Molecular, Facultad de Medicina, Universidad del Rosario, Calle 63 D Número 24–31, Bogotá, Colombia, South America

    Heidy Mateus

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  1. Juan E. Baquero
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  2. Santiago Miranda
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  3. Oscar Murillo
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  4. Heidy Mateus
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  5. Esperanza Trujillo
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  6. Carlos Suarez
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  7. Manuel E. Patarroyo
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  8. Carlos Parra-López
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Correspondence to Carlos Parra-López.

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Baquero, J.E., Miranda, S., Murillo, O. et al. Reference strand conformational analysis (RSCA) is a valuable tool in identifying MHC-DRB sequences in three species of Aotus monkeys. Immunogenetics 58, 590–597 (2006). https://doi.org/10.1007/s00251-006-0101-x

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  • DOI: https://doi.org/10.1007/s00251-006-0101-x

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