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Genotypic Characterization of an MHC Class II Locus in Lake Trout (Salvelinus namaycush) from Lake Superior by Single-Stranded Conformational Polymorphism Analysis and Reference Strand–Mediated Conformational Analysis

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Abstract

This study compares the genotypic information provided by reference strand–mediated conformational analysis and single-stranded confirmational polymorphism (SSCP) analysis for the major histocompatibility complex (MHC) II locus in lake trout. For this study 80 wild-caught animals from the Apostle Islands of Lake Superior were genotyped using both RSCA and SSCP analysis. Their genotypes were recorded using both methods and compared. The genotypic information provided by the 2 methods was essentially the same although some inconsistencies were observed. Both methods detected approximately 65 genotypes, and both were able to distinguish heterozygous and homozygous animals. The analyses determined that only approximately 20% of alleles were shared between 2 morphologically different populations within the sample set, and identified the dominant alleles. SSCP analysis was quicker, simple, and more robust than RSCA. SSCP analysis using fluorescence technologies could be the method of choice for future genotypic analysis of the MHC II locus in salmonids.

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Authors and Affiliations

  1. Washington State University, 14204 NE Salmon Creek Avenue, Vancouver, WA 98686, USA

    Marc A. Noakes & Ruth B. Phillips

  2. Department of Biological Sciences, University of Wisconsin, Milwaukee, Wisconsin, USA

    Tara Reimer

Authors
  1. Marc A. Noakes
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  2. Tara Reimer
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  3. Ruth B. Phillips
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Correspondence to Ruth B. Phillips.

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Noakes, M.A., Reimer, T. & Phillips, R.B. Genotypic Characterization of an MHC Class II Locus in Lake Trout (Salvelinus namaycush) from Lake Superior by Single-Stranded Conformational Polymorphism Analysis and Reference Strand–Mediated Conformational Analysis . Mar. Biotechnol. 5, 270–278 (2003). https://doi.org/10.1007/s10126-002-0079-9

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  • DOI: https://doi.org/10.1007/s10126-002-0079-9

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