Abstract
The identification of C-type lectin (Group V) natural killer (NK) cell receptors in bony fish has remained elusive. Analyses of the Fugu rubripes genome database failed to identify Group V C-type lectin domains (Zelensky and Gready, BMC Genomics 5:51, 2004) suggesting that bony fish, in general, may lack such receptors. Numerous Group II C-type lectin receptors, which are structurally similar to Group V (NK) receptors, have been characterized in bony fish. By searching the zebrafish genome database we have identified a multi-gene family of Group II immune-related, lectin-like receptors (illrs) whose members possess inhibiting and/or activating signaling motifs typical of Group V NK receptors. Illr genes are differentially expressed in the myeloid and lymphoid lineages, suggesting that they may play important roles in the immune functions of multiple hematopoietic cell lineages.
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Notes
Other zebrafish genomic sequences were identified with slightly higher identities to NKG2D but did not appear to encode multi-gene families and were not pursued.
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Acknowledgements
We thank Robert Haire for TCR primers, Jennifer Montgomery for early assistance with RACE and DNA sequencing, Kerstin Howe for genome annotation, and Gary Litman for helpful comments on the manuscript. Jeffrey A. Yoder is supported by funding from the National Science Foundation, National Institute of Health, and a Faculty Research and Professional Development award from North Carolina State University.
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Nucleotide sequence data reported have been deposited in the GenBank and ZFIN databases [accession numbers AY986755 (illr1), AY986756 (illr3_a), AY986757 (illr3_b), and AY986758 (illrL)]. The genome database ID numbers for these genes are (gene/transcript/protein): illr1 (OTTDARG00000014118/ OTTDART00000016839/ OTTDARP00000014272), illr2 (OTTDARG00000014110/ OTTDART 00000016827/ OTTDARP00000017119), illr3_a (OTTDARG00000014108/ OTTDART00000016822/ OTTDARP00000014257), illr3_b (OTTDARG00000014108/ OTTDART00000020282/ OTTDARP00000017118), illrL (OTTDARG00000015418/ OTTDART00000018473/ OTTDARP00000015598)
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Panagos, P.G., Dobrinski, K.P., Chen, X. et al. Immune-related, lectin-like receptors are differentially expressed in the myeloid and lymphoid lineages of zebrafish. Immunogenetics 58, 31–40 (2006). https://doi.org/10.1007/s00251-005-0064-3
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DOI: https://doi.org/10.1007/s00251-005-0064-3