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An Appraisal of Bacteriophage Isolation Techniques from Environment

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Abstract

Researchers have recently renewed interest in bacteriophages. Being valuable models for the study of eukaryotic viruses, and more importantly, natural killers of bacteria, bacteriophages are being tapped for their potential role in multiple applications. Bacteriophages are also being increasingly sought for bacteriophage therapy due to rising antimicrobial resistance among pathogens. Reports show that there is an increasing trend in therapeutic application of natural bacteriophages, genetically engineered bacteriophages, and bacteriophage-encoded products as antimicrobial agents. In view of these applications, the isolation and characterization of bacteriophages from the environment has caught attention. In this review, various methods for isolation of bacteriophages from environmental sources like water, soil, and air are comprehensively described. The review also draws attention towards a handful on-field bacteriophage isolation techniques and the need for their further rapid development.

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Acknowledgements

We are thankful to Director CSIR-NEERI, Nagpur for providing the research facility and infrastructure. We are grateful to Council of Scientific and Industrial Research (CSIR) for funding and supporting the research scholar. The manuscript has been checked for plagiarism using iThenticate Software and assigned KRC No.: CSIR-NEERI/KRC/2019/SEP/EVC/1

Funding

The research scholars Aparna Nair and Gaurav Ghugare were supported for fellowship by Council of Scientific and Industrial Research (CSIR).

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Authors and Affiliations

  1. Environmental Virology Cell, Council of Scientific and Industrial Research-National Environmental Engineering Research Institute, Nehru Marg, Nagpur, 440020, India

    Aparna Nair, Gaurav S. Ghugare & Krishna Khairnar

  2. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India

    Aparna Nair, Gaurav S. Ghugare & Krishna Khairnar

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  1. Aparna Nair
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  2. Gaurav S. Ghugare
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  3. Krishna Khairnar
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AN wrote the manuscript and contributed to conceptualization of the review and its execution. GG contributed to acquisition of data and information relevant to the review. KK conceived and planned the review article and provided critical revision of the final draft.

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Correspondence to Krishna Khairnar.

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Nair, A., Ghugare, G.S. & Khairnar, K. An Appraisal of Bacteriophage Isolation Techniques from Environment. Microb Ecol 83, 519–535 (2022). https://doi.org/10.1007/s00248-021-01782-z

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