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Comparative Analysis of the Intestinal Bacterial Communities in Different Species of Carp by Pyrosequencing

  • Microbiology of Aquatic Systems
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Abstract

Gut microbiota is increasingly regarded as an integral component of the host, due to important roles in the modulation of the immune system, the proliferation of the intestinal epithelium and the regulation of the dietary energy intake. Understanding the factors that influence the composition of these microbial communities is essential to health management, and the application to aquatic animals still requires basic investigation. In this study, we compared the bacterial communities harboured in the intestines and in the rearing water of grass carp (Ctenopharyngodon idellus), crucian carp (Carassius cuvieri), and bighead carp (Hypophthalmichthys nobilis), by using 454-pyrosequencing with barcoded primers targeting the V4 to V5 regions of the bacterial 16S rRNA gene. The specimens of the three species were cohabiting in the same pond. Between 6,218 and 10,220 effective sequences were read from each sample, resulting in a total of 110,398 sequences for 13 samples from gut microbiota and pond water. In general, the microbial communities of the three carps were dominated by Fusobacteria, Firmicutes, Proteobacteria and Bacteroidetes, but the abundance of each phylum was significantly different between species. At the genus level, the overwhelming group was Cetobacterium (97.29 ± 0.46 %) in crucian carp, while its abundance averaged c. 40 and 60 % of the sequences read in the other two species. There was higher microbial diversity in the gut of filter-feeding bighead carp than the gut of the two other species, with grazing feeding habits. The composition of intestine microbiota of grass carp and crucian carp shared higher similarity when compared with bighead carp. The principal coordinates analysis (PCoA) with the weighted UniFrac distance and the heatmap analysis suggested that gut microbiota was not a simple reflection of the microbial community in the local habitat but resulted from species-specific selective pressures, possibly dependent on behavioural, immune and metabolic characteristics.

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Acknowledgements

This work was supported by grants from the National Science and Technology Pillar Programmes (grants no. 2011BAI15B01-41), FEBL project (2011FBZ26) and the Natural Science Foundation of China (no. 30670112 and 31070112).

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Authors and Affiliations

  1. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China

    Tongtong Li, Meng Long, Qianqian Zhang, Aihua Li & Xiaoning Gong

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Tongtong Li, Meng Long & Qianqian Zhang

  3. INRA, UR 1067, Nutrition Metabolism and Aquaculture, Ifremer, Centre de Brest, BP 70, 29280, Plouzané, France

    François-Joël Gatesoupe

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  1. Tongtong Li
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  2. Meng Long
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  3. François-Joël Gatesoupe
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Correspondence to Aihua Li.

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Li, T., Long, M., Gatesoupe, FJ. et al. Comparative Analysis of the Intestinal Bacterial Communities in Different Species of Carp by Pyrosequencing. Microb Ecol 69, 25–36 (2015). https://doi.org/10.1007/s00248-014-0480-8

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