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A Comparison of the Physical and Chemical Differences Between Cancellous and Cortical Bovine Bone Mineral at Two Ages

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An Erratum to this article was published on 11 March 2009

Abstract

To assess possible differences between the mineral phases of cortical and cancellous bone, the structure and composition of isolated bovine mineral crystals from young (1–3 months) and old (4–5 years) postnatal bovine animals were analyzed by a variety of complementary techniques: chemical analyses, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and 31P solid-state magic angle spinning nuclear magnetic resonance spectroscopy (NMR). This combination of methods represents the most complete physicochemical characterization of cancellous and cortical bone mineral completed thus far. Spectra obtained from XRD, FTIR, and 31P NMR all confirmed that the mineral was calcium phosphate in the form of carbonated apatite; however, a crystal maturation process was evident between the young and old and between cancellous and cortical mineral crystals. Two-way analyses of variance showed larger increases of crystal size and Ca/P ratio for the cortical vs. cancellous bone of 1–3 month than the 4–5 year animals. The Ca/(P + CO3) remained nearly constant within a given bone type and in both bone types at 4–5 years. The carbonate and phosphate FTIR band ratios revealed a decrease of labile ions with age and in cortical, relative to cancellous, bone. Overall, the same aging or maturation trends were observed for young vs. old and cancellous vs. cortical. Based on the larger proportion of newly formed bone in cancellous bone relative to cortical bone, the major differences between the cancellous and cortical mineral crystals must be ascribed to differences in average age of the crystals.

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Acknowledgements

We thank Dr. Adele Boskey for her review of this manuscript and Dr. Jon Goldberg for his help with the statistical analysis. Supported in part by National Institutes of Health grants R01-AR34081, R01-AG14701, R01-AR42258, and T32-AR07112; a grant from the Peabody Foundation; the American Foundation for Aging Research; and the Orthopaedic Research and Education Foundation.

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Authors and Affiliations

  1. Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopedic Surgery, Children’s Hospital, Harvard Medical School, Boston, MA, USA

    Liisa T. Kuhn, Yaotang Wu, Jerome L. Ackerman & Melvin J. Glimcher

  2. Department of Reconstructive Sciences, University of Connecticut Health Center, MC1615, Farmington, CT, 06107, USA

    Liisa T. Kuhn

  3. Samuel Lunenfeld Research Institute, Mt. Sinai Hospital, Toronto, Canada

    Marc D. Grynpas

  4. Laboratoire de Physico-Chimie des Solides, Centre National de la Recherche Scientifique, Toulouse, France

    Christian C. Rey

  5. Biomaterials Laboratory, Martinos Center, Department of Radiology, Massachusetts General Hospital, Charlestown, MA, USA

    Yaotang Wu & Jerome L. Ackerman

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  1. Liisa T. Kuhn
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  2. Marc D. Grynpas
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  3. Christian C. Rey
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  4. Yaotang Wu
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  6. Melvin J. Glimcher
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Correspondence to Liisa T. Kuhn.

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An erratum to this article can be found at http://dx.doi.org/10.1007/s00223-009-9234-x

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Kuhn, L.T., Grynpas, M.D., Rey, C.C. et al. A Comparison of the Physical and Chemical Differences Between Cancellous and Cortical Bovine Bone Mineral at Two Ages. Calcif Tissue Int 83, 146–154 (2008). https://doi.org/10.1007/s00223-008-9164-z

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  • DOI: https://doi.org/10.1007/s00223-008-9164-z

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