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X-ray diffraction studies of the crystallinity of bone mineral in newly synthesized and density fractionated bone

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Summary

The crystallinity of bone mineral at different stages of maturation has been measured by quantitative X-ray diffraction methods. Crystallinity measurements were made on tibial middiaphyses from 17-day embryonic chicks, newlyformed periosteal bone from embryonic chicks, and density-fractionated bone from post-hatch chickens from 5 weeks to 2 years of age. For a given animal age and degree of mineralization, crystallinity increases with animal age, indicating that changes in bone mineral occur even after mineralization is complete or nearly complete.

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References

  1. Menczel J, Posner AS, and Harper RA (1965) Age changes in the crystallinity of rat bone apatite. Israel J Med Sci 1:251–252

    PubMed  CAS  Google Scholar 

  2. Burnell JM, Teubner EJ, and Miller AG (1980) Normal maturation changes in bone matrix, mineral, and crystal size in the rat. Calcif Tissue Int 31:13–19

    CAS  PubMed  Google Scholar 

  3. Pellegrino ED, and Biltz RM (1972) Mineralization in the chick embryo I. Monohydrogen phosphate and carbonate relationships during maturation of the bone crystal complex. Calcif Tissue Res 10:128–135

    Article  CAS  PubMed  Google Scholar 

  4. Woodward HQ (1964) The composition of human cortical bone. Clin Orthop 37:187–193

    Google Scholar 

  5. Richelle LJ (1967) Contribution à l'étude du métabolisme mineral de l'os chez le rat. PhD Thesis, University de Liège, Liège

    Google Scholar 

  6. Richelle LJ, and Onkelinx C (1969) Recent advances in the physical biology of bone and other hard tissues. In: Comar C and Bronner F (eds) Mineral metabolism, Vol. III. Academic Press, New York, pp 123–190

    Google Scholar 

  7. Roufosse AH, Landis WJ, Sabine WK, and Glimcher MJ (1979) Identification of brushite in newly deposited bone mineral from embryonic chicks. J Ultrastruct Res 68:235–255

    Article  CAS  PubMed  Google Scholar 

  8. Lian JB, Roufosse AH, Reit B, and Glimcher MJ (1981) The concentrations of osteocalcin and phosphoprotein as a function of mineral content and age in cortical bone. Calcif Tissue Int (in press)

  9. Betts F, Trotta R, Goldberg MR, and Posner AS (1979) Non-apatite mineral in actively calcifying tissue. Trans Orthop Res Soc 4:177

    Google Scholar 

  10. Russell JE, and Avioli LV (1972) Effect of experimental chronic renal insufficiency on bone mineral and collagen maturation. J Clin Invest 51:3072–3079

    Article  CAS  PubMed  Google Scholar 

  11. Dickson IR, and Kodicek E (1979) Effect of Vitamin D deficiency on bone formation in the chick. Biochem J 182:429–435

    CAS  PubMed  Google Scholar 

  12. Eanes ED, Harper RA, Gillessen III, and Posner AS (1966) An amorphous component in bone mineral. In: Gaillard PJ, van der Hoof A, and Steendyk R (eds) Fourth European symposium on calcified tissues. Excerpta Med, Amsterdam pp 24–26

    Google Scholar 

  13. Harper RA, and Posner AS (1966) Measurement of noncrystalline calcium phosphate in bone mineral. Proc Soc Exp Biol Med 122:137–142

    CAS  PubMed  Google Scholar 

  14. Termine JD, and Posner AS (1967) Amorphous/crystalline interrelationships in bone mineral. Calcif Tissue Res 1:8–23

    Article  CAS  PubMed  Google Scholar 

  15. Posner AS, and Betts F (1975) Synthetic amorphous calcium phosphate and its relation to bone mineral structure. Accounts Chem Res 8:273–281

    Article  CAS  Google Scholar 

  16. Grynpas MD, Bonar LC, and Glimcher MJ (1981) Changes in bone mineral with age. Trans Orthop Res Soc 6:16

    Google Scholar 

  17. Grynpas MD, Bonar LC and Glimcher MJ (1982) On the question of amorphous calcium phosphate in bone mineral. In: Veis A (ed) The chemistry and biology of mineralized connective tissues. Elsevier/North Holland, pp 279–283

  18. Glimcher MJ, Bonar LC, Grynpas MD, Landis WJ, and Roufosse AH (1981) Recent studies of bone mineral: is the amorphous calcium phosphate theory valid? J Cryst Growth 53:100–119

    Article  CAS  Google Scholar 

  19. Grynpas MD, Bonar LC, and Glimcher MJ (1981) Absence of amorphous calcium phosphate in bone of various ages: a radial distribution function study. (in preparation)

  20. Posner AS, Betts F, and Blumenthal NC (1979) Bone mineral composition and structure. In: Simmons DJ and Kunin AS (eds) Skeletal research, Academic Press, New York, pp 167–192

    Google Scholar 

  21. Grynpas MD (1976) Crystallinity of bone mineral. J Mat Sci 11:1691–1696

    Article  Google Scholar 

  22. Quinaux N, and Richelle LJ (1967) X-ray diffraction and infrared analysis of bone specific gravity fraction in the growing rat. Israel J Med Sci 3:677–690

    CAS  Google Scholar 

  23. Klug HP, and Alexander LE (1974) X-ray diffraction procedures for polycrystalline and amorphous materials, 2nd edition, John Wiley & Sons, New York, pp 687–692

    Google Scholar 

  24. Dryer RL, Tammes AR, and Rough JI (1957) The determination of phosphorus with N-phenyl-p-phenylenediamine. J Biol Chem 225:177–183

    CAS  PubMed  Google Scholar 

  25. Tanzer ML, and Hunt RD (1964) Experimental lathrysm, an autoradiographic study. J Cell Biol 22:623–631

    Article  CAS  PubMed  Google Scholar 

  26. Landis WJ, Hauschka PV, Rogerson CA, and Glimcher MJ (1977) Electron microscopic observations of bone tissue prepared by ultracryomicrotomy. J Ultrastruct Res 59:185–206

    Article  CAS  PubMed  Google Scholar 

  27. Landis WJ, Paine MC, and Glimcher MJ (1977) Electron microscopic observations of bone tissue prepared anhydrously in organic solvents. J Ultrastruct Res 59:1–30

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Orthopaedic Surgery and the Laboratory for the Study of Skeletal Disorders and Rehabilitation, Harvard Medical School, Children's Hospital Medical Center, 02115, Boston, Massachusetts, USA

    L. C. Bonar, A. H. Roufosse, W. K. Sabine, M. D. Grynpas & M. J. Glimcher

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  1. L. C. Bonar
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  2. A. H. Roufosse
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  3. W. K. Sabine
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  4. M. D. Grynpas
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  5. M. J. Glimcher
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Bonar, L.C., Roufosse, A.H., Sabine, W.K. et al. X-ray diffraction studies of the crystallinity of bone mineral in newly synthesized and density fractionated bone. Calcif Tissue Int 35, 202–209 (1983). https://doi.org/10.1007/BF02405032

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