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Multiplex real-time PCR for the detection and quantification of DNA from beef, pork, horse and sheep

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Abstract

Meat products are often composed of more than one meat species. A quantitative multiplex PCR was developed to determine the proportion of meat fractions of beef, pork, horse and sheep. The precision and accuracy were investigated by dilutions of DNA from all four species and examining different meat products from the market. Application of this tetraplex quantitative real-time PCR system will enable official food control and production control laboratories to efficiently investigate the composition of meat products.

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Acknowledgments

We thank the cantonal laboratory of Zürich, the Official Food Control Authority of the Canton Thurgau and the Swiss Quality Testing Services (SQTS) Courtepin for providing the resources for this work.

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Authors and Affiliations

  1. Official Food Control Authority of the Canton Thurgau, Thurgau, Switzerland

    Jürg Ruf

  2. Official Food Control Authority of the Canton Zürich, Zürich, Switzerland

    René Köppel

  3. Swiss Quality Testing Services (SQTS), Courtepin, Switzerland

    Jürg Rentsch

Authors
  1. René Köppel
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  2. Jürg Ruf
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  3. Jürg Rentsch
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Correspondence to René Köppel.

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Köppel, R., Ruf, J. & Rentsch, J. Multiplex real-time PCR for the detection and quantification of DNA from beef, pork, horse and sheep. Eur Food Res Technol 232, 151–155 (2011). https://doi.org/10.1007/s00217-010-1371-y

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  • DOI: https://doi.org/10.1007/s00217-010-1371-y

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