Skip to main content
SpringerLink
Log in

Use of pneumatic nebulization and laser ablation–inductively coupled plasma–mass spectrometry to study the distribution and bioavailability of an intraperitoneally administered Pt-containing chemotherapeutic drug

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

Quadrupole-based inductively coupled–mass spectrometry (ICP-MS) with pneumatic nebulization as a means of sample introduction was employed for quantification of platinum in blood and tissue samples of rats with peritoneal carcinomatosis, receiving intraperitoneal treatment with the Pt-containing chemotherapeutic drug oxaliplatin, and in the perfusate solution used for this purpose. The Pt levels were measured for various treatment conditions, i.e., with and without supporting treatment with the drug bevacizumab and at two different temperatures. Limits of detection obtained for platinum in blood and tissue samples were 0.3 and 2.0 pg g −1, respectively. Evaluation of drug penetration into the tumor, under different conditions of treatment, was carried out via laser ablation–ICP-MS. Quantitative mapping of the Pt distribution in tissue sections of rat was attempted relying on gelatin standards. The results show an influence of the temperature at which the treatment is carried out, while supporting administration of the drug bevacizumab did not seem to affect the results.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Stewart JH, Shen P, Levine EA (2005) Ann Surg Oncol 12:765–777

    Article  Google Scholar 

  2. Verwaal VJ, van Ruth S, de Bree E, van Slooten GW, van Tinteren H, Boot H, Zoetmulder FAN (2003) J Clin Oncol 21:3737–3743

    Article  Google Scholar 

  3. Sugarbaker PH (2004) Ann Med (Stockholm, Swed) 36:9–22

    Google Scholar 

  4. Yang X, Huang C, Suo T, Mei L, Yang G, Cheng F, Zhou Y, Xiong B, Yonemura Y, Li Y (2011) Ann Surg Oncol 18:1575–1581

    Article  Google Scholar 

  5. Di Giorgio A, Naticchioni E, Biacchi D, Sibio S, Accarpio F, Rocco M, Tarquini S, Di Seri M, Ciardi A, Montruccoli D, Sammartino P (2008) Cancer 113:315–325

    Article  Google Scholar 

  6. Ceelen W, van Nieuwenhove Y, van Belle S, Denys H, Pattyn P (2010) Ann Surg Oncol 17:S90–S91

    Article  Google Scholar 

  7. Klaver YLB, Hendriks T, Lomme RMLM, Rutten HJT, Bleichrodt RP, de Hingh IHJT (2011) Ann Surg 254:125–130

    Article  Google Scholar 

  8. Roviello F, Caruso S, Marrelli D, Pedrazzani C, Neri A, De Stefano A, Pinto E (2011) Surg Oncol 20:e38–e54

    Article  Google Scholar 

  9. Morrison JG, White P, McDougall S, Firth JW, Woolfrey SG, Graham MA, Greenslade D (2000) J Pharm Biomed Anal 24:1–10

    Article  CAS  Google Scholar 

  10. De Muynck D, Vanhaecke F (2009) Spectrochim Acta Part B 64:408–415

    Article  Google Scholar 

  11. Zimmermann S, Menzel CM, Berner Z, Eckhardt JD, Stüben D, Alt F, Messerschmidt J, Taraschewski H, Sures B (2001) Anal Chim Acta 439:203–209

    Article  CAS  Google Scholar 

  12. Hare D, Tolmachev S, James A, Bishop D, Austin C, Fryer F, Doble P (2010) Anal Chem 82:3176–3182

    Article  CAS  Google Scholar 

  13. Jackson B, Harper S, Smith L, Flinn J (2006) Anal Bioanal Chem 384:951–957

    Article  CAS  Google Scholar 

  14. Feldmann J, Kindness A, Ek P (2002) J Anal At Spectrom 17:813–818

    Article  CAS  Google Scholar 

  15. Matusch A, Depboylu C, Palm C, Wu B, Hoeglinger GU, Schaefer MK, Becker JS (2010) J Am Soc Mass Spectrom 21:161–171

    Article  CAS  Google Scholar 

  16. Lobinski R, Moulin C, Ortega R (2006) Biochimie 88:1591–1604

    Article  CAS  Google Scholar 

  17. Heeren RMA, McDonnell LA, Amstalden E, Luxembourg SL, Altelaar AFM, Piersma SR (2006) Appl Surf Sci 252:6827–6835

    Article  CAS  Google Scholar 

  18. Gholap DS, Izmer A, De Samber B, van Elteren JT, Šelih VS, Evens R, De Schamphelaere K, Janssen C, Balcaen L, Lindemann I, Vincze L, Vanhaecke F (2010) Anal Chim Acta 664:19–26

    Article  CAS  Google Scholar 

  19. Becker JS, Zoriy M, Su BJ, Dobrowolska J, Matusch A (2007) J Anal At Spectrom 22:736–744

    Article  CAS  Google Scholar 

  20. Kang D, Amarasiriwardena D, Goodman AH (2004) Anal Bioanal Chem 378:1608–1615

    Article  CAS  Google Scholar 

  21. Hare D, Austin C, Doble P, Arora M (2011) J Dent (Oxford, UK) 39:397–403

    CAS  Google Scholar 

  22. Fricker MB, Kutscher D, Aeschlimann B, Frommer J, Dietiker R, Bettmer J, Günther D (2011) Int J Mass Spectrom 307:39–45

    CAS  Google Scholar 

  23. Roos PH, Venkatachalam A, Manz A, Waentig L, Koehler CU, Jakubowski N (2008) Anal Bioanal Chem 392:1135–1147

    Article  CAS  Google Scholar 

  24. Zoriy M, Matusch A, Spross T, Becker JS (2007) Int J Mass Spectrom 260:102–106

    Article  CAS  Google Scholar 

  25. Pozebon D, Dressler VL, Matusch A, Becker JS (2008) Int J Mass Spectrom 272:57–62

    Article  CAS  Google Scholar 

  26. Moreno-Gordaliza E, Giesen C, Lazaro A, Esteban-Fernandez D, Humanes B, Canas B, Panne U, Tejedor A, Jakubowski N, Gomez-Gomez M (2011) Anal Chem 83:7933–7940

    Article  CAS  Google Scholar 

  27. Miller JC, Miller JN (1988) Statistics for Analytical Chemistry. Ellis Horwood Ltd, Chichester

    Google Scholar 

  28. Triglav J, van Elteren JT, Šelih VS (2010) Anal Chem 82:8153–8160

    Article  CAS  Google Scholar 

Download references

Acknowledgment

The authors would like to thank Ghent University “Concerted Research Action” (GOA) fund for providing financial support.

Author information

Authors and Affiliations

  1. Department of Analytical Chemistry, Ghent University, Krijgslaan 281-S12, 9000, Ghent, Belgium

    Deepti Gholap & Frank Vanhaecke

  2. Department of Surgery, Ghent University Hospital, De Pintelaan 185, 9000, Ghent, Belgium

    Johanna Verhulst & Wim Ceelen

Authors
  1. Deepti Gholap
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Johanna Verhulst
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wim Ceelen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Frank Vanhaecke
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Frank Vanhaecke.

Electronic supplementary material

Below is the link to the electronic supplementary material.

ESM 1

(PDF 110 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gholap, D., Verhulst, J., Ceelen, W. et al. Use of pneumatic nebulization and laser ablation–inductively coupled plasma–mass spectrometry to study the distribution and bioavailability of an intraperitoneally administered Pt-containing chemotherapeutic drug. Anal Bioanal Chem 402, 2121–2129 (2012). https://doi.org/10.1007/s00216-011-5654-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-011-5654-3

Keywords

Search

Navigation