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Elemental mapping and quantitative analysis of Cu, Zn, and Fe in rat brain sections by laser ablation ICP-MS

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Abstract

This report details the application of laser ablation quadrupole ICP-MS for the (multi)elemental mapping of 100-μm-thick sections of rat brain. The laser spot size used was 60 μm, and the laser scan speed was 120 μm s−1. The analysis was relatively rapid, allowing mapping of a whole brain thin section (≈1 cm2) in about 2 h. Furthermore, the method was amenable to multi-element data collection including the physiologically important elements P and S and afforded sub μg g−1 detection limits for the important trace elements Cu and Zn. Calibrations were performed with pressed pellets of biological certified reference materials, and the elemental distributions and concentrations of Cu, Zn, and Fe were determined in whole rat brain sections. The distributions and concentration ranges for these elements were consistent with previous studies and demonstrate the utility of this technique for rapid mapping of brain thin sections.

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Acknowledgements

We would like to thank Ann Butler, Blair Jones and Robert Switzer for helpful discussions and Katherine Cano and Andrea Fitzgerald for technical assistance. This research was supported by the Environmental Remediation Sciences Division of the Office of Biological and Environmental Research, US DOE through the financial assistance award DE-FC09-96SR18546 to the University of Georgia Research Foundation. Manuscript preparation was partially supported by by NIH Grant Number P42 ESO7373 from the National Institute of Environmental Health Sciences.

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Authors and Affiliations

  1. Departments of Earth Sciences and Chemistry, Dartmouth College, Hanover, NH, 03755, USA

    Brian Jackson

  2. Savannah River Ecology Laboratory, University of Georgia, Aiken, SC, 29802, USA

    Steve Harper

  3. Department of Psychology, George Mason University, 4400 University Drive, Fairfax, VA, 22030, USA

    Laura Smith & Jane Flinn

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  1. Brian Jackson
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  2. Steve Harper
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  3. Laura Smith
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  4. Jane Flinn
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Correspondence to Brian Jackson.

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Jackson, B., Harper, S., Smith, L. et al. Elemental mapping and quantitative analysis of Cu, Zn, and Fe in rat brain sections by laser ablation ICP-MS. Anal Bioanal Chem 384, 951–957 (2006). https://doi.org/10.1007/s00216-005-0264-6

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  • DOI: https://doi.org/10.1007/s00216-005-0264-6

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