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Collagen types analysis and differentiation by FTIR spectroscopy

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Abstract

Abnormal formation and organization of collagen network is commonly observed in many organ pathologies, but analytical techniques able to reveal the collagen biodistribution are still lacking. In this study, Fourier-transform infrared (FTIR) spectroscopy has been used to analyze type I, III, IV, V, and VI collagens, the most important compounds of connective tissues. A robust classification of 30 FTIR spectra per collagen type could be obtained by using a combination of four spectral intervals [ν(C=O) absorption of amide I (1,700–1,600 cm−1), δ(CH2), and δ(CH3) absorptions (1,480–1,350 cm−1), ν(C–N), and δ(N–H) absorptions of amide III (1,300–1,180 cm−1), and ν(C–O) and ν(C–O–C) absorptions of carbohydrate moieties (1,100–1,005 cm−1)]. Then, a submolecular justification of this classification model was sought using a curve fitting analysis of the four spectral intervals. Results demonstrated that every spectral interval used for the classification contained highly discriminant absorption bands between all collagen types (multivariate analysis of variance, p < 0.01; Dunnett's T3 post hoc test, p < 0.05). All conditions seem thus joined to make FTIR spectroscopy and imaging major tools for implementing innovative methods in the field of molecular histology, which would be very helpful for the diagnosis of a wide range of pathologies.

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Abbreviations

FTIR:

Fourier-transform infrared

RMSE:

Root mean square error

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Acknowledgment

The authors are indebted to the “Association Française contre les Myopathies” (AFM) for their financial support.

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Authors and Affiliations

  1. Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076, Bordeaux-Cedex, France

    Karima Belbachir, Razia Noreen, Gilles Gouspillou & Cyril Petibois

  2. CNRS UMR 5248, CBMN, 2 rue Robert Escarpit, 33607, Pessac-Cedex, France

    Karima Belbachir, Razia Noreen & Cyril Petibois

Authors
  1. Karima Belbachir
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  2. Razia Noreen
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  3. Gilles Gouspillou
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  4. Cyril Petibois
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Corresponding author

Correspondence to Cyril Petibois.

Electronic supplementary material

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ESM Table S1–S4

Table S1: Secondary structure parameters of collagen types. All values are spectral integration results obtained from amides I and II (1,720–1,480 cm−1) spectral interval curve fitting. a = significantly different from type I collagen; b = significantly different from type III collagen; c = significantly different from type IV collagen; d = significantly different from type V collagen; e = significantly different from type VI collagen. Table S2: IR absorption bands obtained from the 1,500–1,300 cm−1 spectral interval curve fitting. See supplementary data 1 for details on statistics. Table S3: IR absorption bands obtained from the 1,350–1,150 cm−1 spectral interval curve fitting. See Table S1 for details on statistics. Table S4: IR absorption bands obtained from the 1,130–950 cm−1 spectral interval curve fitting. See Table S1 for details on statistics. (PDF 58 kb)

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Belbachir, K., Noreen, R., Gouspillou, G. et al. Collagen types analysis and differentiation by FTIR spectroscopy. Anal Bioanal Chem 395, 829–837 (2009). https://doi.org/10.1007/s00216-009-3019-y

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  • DOI: https://doi.org/10.1007/s00216-009-3019-y

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