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FT-IR spectral imaging of blood vessels reveals protein secondary structure deviations induced by tumor growth

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Abstract

Vascular basement membrane remodeling is involved in tumor angiogenesis to enable tumor invasion and growth. FT-IR spectral imaging was used to determine changes in tumor blood vessels to reveal protein secondary structure in Rag-gamma immuno-deficient mice sacrificed 14 and 21 days after subcutaneous glioma implantation. For the oldest blood capillaries (diameter >20 microns), tumor growth induced a decrease in triple-helix content (1638 cm−1; −7.3%; P < 0.05) and an increase in beta turns (1666 and 1615 cm−1; +4%; P < 0.01). These protein-structure alterations, mainly from type IV collagen, reflected the high angiogenic stress of growing tumors. We propose to use these molecular markers of vascular basement membrane protein alterations for gradation of solid tumors by FT-IR spectral imaging.

From spectral curve fitting to tumor signature by blood capillaries FT-IR imaging was used to analyze vascular basement membrane protein contents changes along tumor growth. Changes in protein secondary structure (decrease in triple helix and increase in β-turns contents) revealed the high angiogenic stress of growing tumors. These structural changes might be used as molecular markers for a functional FT-IR imaging of tumor growth. FT-IR imaging of a tumor blood capillary. Spectral curve-fitting of pixels corresponding to vascular basement membrane of the capillary is used to determine secondary structure of protein contents.

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Abbreviations

BM:

Basement membrane

DMEM:

Dulbecco’s modified Eagle’s medium

FBS:

Fetal bovine serum

FT-IR:

Fourier-transform infrared

MRI:

Magnetic resonance imaging

PET:

Positron-emission tomography

VBM:

Vascular basement membrane

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Authors and Affiliations

  1. Université de Bordeaux, CNRS UMR 5084, CNAB, 146 Rue Léo Saignat, 33076, Bordeaux-Cedex, France

    Katia Wehbe, Gérard Déléris & Cyril Petibois

  2. Université Bordeaux 1, INSERM U920, Avenue des Facultés, 33405, Talence-Cedex, France

    Raphael Pinneau & Michel Moenner

Authors
  1. Katia Wehbe
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  2. Raphael Pinneau
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  3. Michel Moenner
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  4. Gérard Déléris
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  5. Cyril Petibois
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Correspondence to Cyril Petibois.

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Wehbe, K., Pinneau, R., Moenner, M. et al. FT-IR spectral imaging of blood vessels reveals protein secondary structure deviations induced by tumor growth. Anal Bioanal Chem 392, 129–135 (2008). https://doi.org/10.1007/s00216-008-2230-6

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  • DOI: https://doi.org/10.1007/s00216-008-2230-6

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