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Label-free detection of cancer biomarker candidates using surface plasmon resonance imaging

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Abstract

In this work, we present an antibody array for the detection of cancer biomarker candidates by a surface plasmon resonance (SPR) imaging sensor with polarization contrast. Responses from the SPR imaging sensor are shown to be similar to those from a conventional spectroscopy-based SPR sensor. Antibodies are spotted onto a self-assembled monolayer (SAM) composed of oligo(ethylene glycol) (OEG)-containing alkanethiol chains. Detection of two cancer biomarker candidates, activated leukocyte cell adhesion molecule/CD 166 (ALCAM) and transgelin-2 (TAGLN2), is demonstrated. Limits of detection for ALCAM and TAGLN2 are established at 6 ng/mL and 3 ng/mL, respectively, in buffer. No cross-reactivity is observed between immobilized antibodies and nonspecific antigen. Biomarker candidates are also detected in a 10% human serum solution.

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References

  1. Wulfkuhle JD, Liotta LA, Petricoin EF (2003) Nat Rev Cancer 3:267–275

    Article  CAS  Google Scholar 

  2. Etzioni R, Urban N, Ramsey S, McIntosh M, Schwartz S, Reid B, Radich J, Anderson G, Hartwell L (2003) Nat Rev Cancer 3:243–252

    Article  CAS  Google Scholar 

  3. Calvo KR, Liotta LA, Petricoin EF (2005) Biosci Rep 25:107–125

    Article  CAS  Google Scholar 

  4. Marrero JA, Lok ASF (2004) Gastroenterology 127:S113–S119

    Article  CAS  Google Scholar 

  5. Mikolajczyk SD, Song Y, Wong JR, Matson RS, Rittenhouse HG (2004) Clin Biochem 37:519–528

    Article  CAS  Google Scholar 

  6. Burkhardt M, Mayordomo E, Winzer KJ, Fritzsche F, Gansukh T, Pahl S, Weichert W, Denkert C, Guski H, Dietel M, Kristiansen G (2006) J Clin Pathol 59:403–409

    Article  CAS  Google Scholar 

  7. Weichert W, Knosel T, Bellach J, Dietel M, Kristiansen G (2004) J Clin Pathol 57:1160–1164

    Article  CAS  Google Scholar 

  8. Shi YY, Wang HC, Yin YH, Sun WS, Li Y, Zhang CQ, Wang Y, Wang S, Chen WF (2005) Br J Cancer 92:929–934

    Article  CAS  Google Scholar 

  9. Shields JM, Rogers-Graham K, Der CJ (2002) J Biol Chem 277:9790–9799

    Article  CAS  Google Scholar 

  10. Quek ML, Sanderson K, Daneshmand S, Stein JP (2004) Curr Opin Urol 14:259–264

    Article  Google Scholar 

  11. Liotta LA, Espina V, Mehta AI, Calvert V, Rosenblatt K, Geho D, Munson PJ, Young L, Wulfkuhle J, Petricoin EF (2003) Cancer Cell 3:317–325

    Article  CAS  Google Scholar 

  12. Nelson BP, Grimsrud TE, Liles MR, Goodman RM, Corn RM (2001) Anal Chem 73:1–7

    Article  CAS  Google Scholar 

  13. Piliarik M, Vaisocherova H, Homola J (2005) Biosens Bioelectron 20:2104–2110

    Article  CAS  Google Scholar 

  14. Steinerg G, Sablinskas V, Hubner A, Kuhne C, Salzer R (1999) J Mol Struct 509:265–273

    Article  Google Scholar 

  15. Usui-Aoki K, Shimada K, Nagano M, Kawai M, Koga H (2005) Proteomics 5:2396–2401

    Article  CAS  Google Scholar 

  16. Lee SJ, Youn BS, Park JW, Niazi JH, Kim YS, Gu MB (2008) Anal Chem 80:2867–2873

    Article  CAS  Google Scholar 

  17. Wassaf D, Kuang GN, Kopacz K, Wu QL, Nguyen Q, Toews M, Cosic J, Jacques J, Wiltshire S, Lambert J, Pazmany CC, Hogan S, Ladner RC, Nixon AE, Sexton DJ (2006) Anal Biochem 351:241–253

    Article  CAS  Google Scholar 

  18. Klenkar G, Liedberg B (2008) Anal Bioanal Chem 391:1679–1688

    Article  CAS  Google Scholar 

  19. Lee HJ, Wark AW, Corn RM (2008) Analyst 133:596–601

    Article  CAS  Google Scholar 

  20. Ladd J, Taylor AD, Piliarik M, Homola J, Jiang S (2008) Anal Chem 80:4231–4236

    Article  CAS  Google Scholar 

  21. Piliarik M, Katainen J, Homola J (2007) Proc SPIE 658515

  22. Piliarik M, Homola J (2008) Sensor Actuat B-Chem 134:353–355

    Article  Google Scholar 

  23. Campbell CT, Kim G (2007) Biomaterials 28:2380–2392

    Article  CAS  Google Scholar 

  24. Chinowsky TM, Grow MS, Johnston KS, Nelson K, Edwards T, Fu E, Yager P (2007) Biosens Bioelectron 22:2208–2215

    Article  CAS  Google Scholar 

  25. Fu E, Chinowsky T, Foley J, Weinstein J, Yager P (2004) Rev Sci Instrum 75:2300–2304

    Article  CAS  Google Scholar 

  26. Fang Y, Ferrie A, Lai F (2005) Production of protein microarrays using robotic pin printing technologies. In: Walker J (ed) The proteomics protocols handbook. Humana, New Jersey

    Google Scholar 

  27. Thomsen V, Schatzlein D, Mercuro D (2003) Spectroscopy 18:112–114

    CAS  Google Scholar 

  28. Faca VM, Song KS, Wang H, Zhang Q, Krasnoselsky AL, Newcomb LF, Plentz RR, Gurumurthy S, Redston MS, Pitteri SJ, Pereira-Faca SR, Ireton RC, Katayama H, Glukhova V, Phanstiel D, Brenner DE, Anderson MA, Misek D, Scholler N, Urban ND, Barnett MJ, Edelstein C, Goodman GE, Thornquist MD, McIntosh MW, DePinho RA, Bardeesy N, Hanash SM (2008) PLoS Med 5:953–967

    Article  Google Scholar 

  29. Kyo M, Usui-Aoki K, Koga H (2005) Anal Chem 77:7115–7121

    Article  CAS  Google Scholar 

  30. Masson JF, Battaglia TM, Khairallah P, Beaudoin S, Booksh KS (2007) Anal Chem 79:612–619

    Article  CAS  Google Scholar 

  31. Phillips KS, Han JH, Cheng Q (2007) Anal Chem 79:899–907

    Article  CAS  Google Scholar 

  32. Ladd J, Zhang Z, Chen S, Hower JC, Jiang S (2008) Biomacromolecules 9:1357–1361

    Article  CAS  Google Scholar 

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Acknowledgements

The authors would like to thank Dr. Samir Hanash at the Fred Hutchinson Cancer Research Center and Dr. Hana Vaisocherova at the University of Washington for helpful discussions regarding cancer biomarkers and biomarker candidates. This work was supported by the National Science Foundation (CBET-0528605).

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Washington, Seattle, WA, 98195, USA

    Jon Ladd, Allen D. Taylor & Shaoyi Jiang

  2. Institute of Photonics and Electronics of the Academy of Sciences of the Czech Republic, 182 51, Prague, Czech Republic

    Marek Piliarik & Jiri Homola

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  1. Jon Ladd
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  2. Allen D. Taylor
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  3. Marek Piliarik
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  4. Jiri Homola
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  5. Shaoyi Jiang
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Corresponding author

Correspondence to Shaoyi Jiang.

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Ladd, J., Taylor, A.D., Piliarik, M. et al. Label-free detection of cancer biomarker candidates using surface plasmon resonance imaging. Anal Bioanal Chem 393, 1157–1163 (2009). https://doi.org/10.1007/s00216-008-2448-3

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  • DOI: https://doi.org/10.1007/s00216-008-2448-3

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