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Direct electrochemistry and electrochemical catalysis of immobilized hemoglobin in an ethanol–water mixture

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Abstract

Hemoglobin (Hb) was immobilized on a glassy carbon electrode (GCE) surface by konjac glucomannan (KGM). KGM hydrogel films on GCE have relatively high stabilities in aqueous–ethanol mixtures. The entrapped hemoglobin undergoes fast direct electron transfer reactions in aqueous–organic solvent mixtures. The peak current is bigger, the peak-to-peak separation smaller and the formal potential observed in the cyclic voltammogram is more negative for Hb–KGM/GCE in ethanol–PBS compared to Hb–KGM/GCE in PBS. The electrochemical properties of the Hb in aqueous–organic solution are almost unchanged from with those observed for the purely aqueous solution, suggesting that water pools in the KGM hydrogel play an important role in preventing changes in conformation and making proteins unreactive with polar organic solvents. The immobilized Hb was able to catalyze the reduction of nitric oxide, peroxides (hydrogen peroxide, cumene hydroperoxide, t-butyl hydroperoxide, 2-butanone peroxide), and the dehalogenation of haloethanes (hexachloroethane, pentachloroethane, tetrachloroethane, etc.). The stability and reproducibility of the modified electrode meant that it could be used to determine these substances.

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Acknowledgements

This work was supported by the China Postdoctoral Science Foundation (20040350605), the Natural Science Foundation of Hubei Province (2005ABA007), and the Fund from the Education Department of Hubei Province, China (T20030718, G200525001).

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Authors and Affiliations

  1. College of Life Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China

    Hui-Hong Liu & Guo-Lin Zou

  2. Department of Chemistry and Biology, Xiangfan University, Xiangfan, 441053, People’s Republic of China

    Hui-Hong Liu & Yong-Qing Wan

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  1. Hui-Hong Liu
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  2. Yong-Qing Wan
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Correspondence to Guo-Lin Zou.

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Liu, HH., Wan, YQ. & Zou, GL. Direct electrochemistry and electrochemical catalysis of immobilized hemoglobin in an ethanol–water mixture. Anal Bioanal Chem 385, 1470–1476 (2006). https://doi.org/10.1007/s00216-006-0588-x

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  • DOI: https://doi.org/10.1007/s00216-006-0588-x

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