Skip to main content
SpringerLink
Log in

Determination of magnesium, calcium, sodium, and potassium in blood plasma samples by capillary zone electrophoresis

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

A capillary zone electrophoretic assay has been developed and validated for analysis of magnesium, calcium, sodium, and potassium in blood plasma samples. Optimum results were obtained with 20 mmol L−1 imidazole (pH 2.8) and 0.5 mmol L−1 oxalic acid containing 5% methanol, capillary temperature 25°C, applied voltage 30 kV, hydrodynamic injection time 3 s, and a poly(vinyl alcohol)-coated capillary (i.d. 50 μm, total length 64.5 cm and effective length 56 cm). Indirect detection was performed at 214 nm. Cadmium was used as internal standard. The migration times of magnesium, calcium, sodium, and potassium were 4.25, 3.79, 3.96, and 2.79 min, respectively. The method was applied to the determination of magnesium, calcium, sodium, and potassium in blood plasma samples. The results were compared with those from atomic absorption spectrophotometry and no statistically significant difference was found (P>0.05).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Timerbaev AR, Fukushi K (2003) Mar Chem 82:221–238

    Article  CAS  Google Scholar 

  2. Sirén H, Kokkonen R, Hiissa T, Särme T, Rimpinen O, Laitinen R (2000) J Chromatogr, A 895:189–196

    Article  Google Scholar 

  3. Yi Z, Zhuang G, Brown PR (1993) J Chromatogr, A 16:3133–3170

    CAS  Google Scholar 

  4. Marumo Y, Inoue T, Seta S (1994) Forensic Sci Int 69:89–95

    Article  CAS  Google Scholar 

  5. Turner DR, Varney MS, Whitfield M, Mantoura RFC, Riley J P (1987) Sci Total Environ:17–34

    Article  CAS  Google Scholar 

  6. de Paz E, Rabanal B, Negro A (1998) J Liq Chromatogr Related Technol 21:2589–2605

    Article  Google Scholar 

  7. Qu F, Lin JM, Chen Z (2004) J Chromatogr A 1022:217–221

    Article  PubMed  CAS  Google Scholar 

  8. Kuban P, Oldhoff O, Karlberg B (1999) J Chromatogr A 857:321–326

    Article  PubMed  CAS  Google Scholar 

  9. Huang X, Pang TJ, Gordon M, J Zare RN (1987) Anal Chem 59:2747–2749

    Article  CAS  Google Scholar 

  10. Altria KD, Wallberg M, Westerlun DJ (1998) Chromatogr B 714:99–104

    Article  CAS  Google Scholar 

  11. Kubáň P, Kubáň P, Kubáň V (1999) J Chromatogr, A 836:75–80

    Article  Google Scholar 

  12. Steiner F, Beck W, Engelhardt H (1996) J Chromatogr, A 738:11–23

    Article  CAS  Google Scholar 

  13. Lin TI, Lee YH, Chen YC (1993) J Chromatogr, A 654:167–176

    Article  CAS  Google Scholar 

  14. Daali Y, Cherkaoui S, Christen P, Veuthey J (1999) Electrophoresis 20:3424–3431

    Article  PubMed  CAS  Google Scholar 

  15. Baker DR (1995) Capillary electrophoresis. Wiley, New York

    Google Scholar 

  16. Cahours X, Morin P, Dreux M (1998) J Chromatogr, A 810:209–220

    Article  CAS  Google Scholar 

  17. Chamberlain J (1995) The analysis of drugs in biological fluids. CRC, Boca Raton

    Google Scholar 

  18. ICH Topic Q2A Validation of Analytical Procedures: Methodology CPMP/ICH/281/95

  19. Fabre H, Altria KD (2001) LC-GC 14:302–310

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Pharmacy, University of Hacettepe, 06100, Ankara, Turkey

    Emirhan Nemutlu & Nuran Özaltın

Authors
  1. Emirhan Nemutlu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nuran Özaltın
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Nuran Özaltın.

Additional information

This study was supported by the Turkish Republic, Prime Ministry State Planning Organization (Project Number: 98K121730)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nemutlu, E., Özaltın, N. Determination of magnesium, calcium, sodium, and potassium in blood plasma samples by capillary zone electrophoresis. Anal Bioanal Chem 383, 833–838 (2005). https://doi.org/10.1007/s00216-005-0063-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-005-0063-0

Keywords

Search

Navigation