Skip to main content
SpringerLink
Log in

Polypyrrole modified stainless steel frits for on-line micro solid phase extraction of ochratoxin A

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

Polypyrrole (PPy) was electrochemically synthesized on stainless steel frits as a sorbent for the micro solid phase extraction (μSPE) of ochratoxin A (OTA). Using 20 μl of standard solution under a fast flow rate of 0.5 ml/min, 80% recovery of OTA was achieved in the concentration range from 0.1–10 pg/μl. This good recovery was achieved within a short residence time of 1.2 s. A binding capacity of 1 ng OTA was estimated for each PPy-modified frit, or 2 ng OTA for two frits in series. The bound OTA could be pulsed eluted (PE) with 20 μl of 1% triethylamine in acetonitrile. On-line coupling of this PPy-on-a-frit and PE technique to high performance liquid chromatography (HPLC) was straightforward. On-line μSPE-PE-HPLC results clearly demonstrated the capability of PPy-on-a-frit to bind OTA in the presence of red wine, beer, and orange juice components.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Wu J, Yu X, Lord H, Pawliszyn J (2000) Analyst 125:391–394

    Article  CAS  Google Scholar 

  2. Wang LX, Li XG, Yang YL (2001) React Funct Polym 47:125–139

    Article  Google Scholar 

  3. Khalkhali RA, Price WE, Wallace GG (2003) React Funct Polym 56:141–146

    Article  Google Scholar 

  4. Sadki S, Schottland P, Brodie N, Sabouraud G (2000) Chem Soc Rev 29:283–293

    Google Scholar 

  5. Chen F, Shi G, Fu M, Qu L, Hong X (2003) Synthetic Met 132:125–132

    Google Scholar 

  6. Wu J, Pawliszyn J (2001) J Chromatogr A 909:37–52

    Article  CAS  PubMed  Google Scholar 

  7. Liljegren G, Pettersson J, Markides KE, Nyholm L (2002) Analyst 127:591–597

    Article  Google Scholar 

  8. Wu J, Mester Z, Pawliszyn J (2000) Anal Chim Acta 424:211–222

    Article  Google Scholar 

  9. Bagheri H, Mohammadi A (2003) J Chromatogr A 1015:23–30

    Article  CAS  PubMed  Google Scholar 

  10. Wu J, Lord H, Pawliszyn J (2001) Talanta 54:655–672

    Article  Google Scholar 

  11. Yu JCC, Lai EPC (2004) Synthetic Met 143:253–258

    Google Scholar 

  12. Qu L, Shi G, Yuan J, Han G, Chen F (2004) J Electroanal Chem 561:149–156

    Article  Google Scholar 

  13. Alltech (2004) Chromatography sourcebook: catalog. Alltech Associates, Deerfield, IL, 550:572–573

  14. Li KM, Thompson MR, McGregor IS (2004) J Chromatogr B 804:319–326

    Article  Google Scholar 

  15. Viberg P, Nilsson S, Skog K (2004) Anal Bioanal Chem 378:1729–1734

    Article  Google Scholar 

  16. Monaci L, Palmisano F (2004) Anal Bioanal Chem 378:96–103

    Article  Google Scholar 

  17. Jornet D, Busto O, Guasch J (2000) J Chromatogr A 882:29–35

    Article  Google Scholar 

  18. Valenta H (1998) J Chromatogr A 815:75–92

    Article  Google Scholar 

  19. Domijan AM, Peraica M, Miletic-Medved M, Lucic A, Fuchs R (2003) J Chromatogr B 798:317–321

    Article  Google Scholar 

  20. González-Peñas E, Leache C, Viscarret M, Pérez de Obanos A, Araguás C, López de Cerain A (2004) J Chromatogr A 1025:163–168

    Article  Google Scholar 

  21. International Conference on Harmonization (1995) Draft guideline on validation of analytical procedures: definitions and terminology. Federal Register 60:11260

    Google Scholar 

  22. Dall’Asta C, Galaverna G, Dossena A, Marchelli R (2004) J Chromatogr A 1024:275–279

    Article  Google Scholar 

  23. Stein Carter J (2000) Webpage showing pH Testing results. See http://biology.clc.uc.edu/scripts/pHResl.pl

  24. Zimmerli B, Dick R (1995) J Chromatogr B 666:85–99

    Google Scholar 

  25. Sáez JM, Medina Á, Gimeno-Adelantado JV, Mateo R, Jiménez M (2004) J Chromatogr A 1029:125–133

    Article  Google Scholar 

  26. Aresta A, Cioffi N, Palmisano F, Zambonin CG (2003) J Agr Food Chem 51:5232–5237

    Google Scholar 

  27. Jodlbauer J, Maier NM, Lindner W (2002) J Chromatogr A 945:45–63

    Article  Google Scholar 

  28. Zhou SNS, Lai EPC, Miller JD (2004) Anal Bioanal Chem 378:1903–1906

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

Financial support of the Natural Sciences and Engineering Research Council (NSERC) Canada is gratefully acknowledged.

Author information

Authors and Affiliations

  1. Ottawa-Carleton Chemistry Institute, Department of Chemistry, Carleton University, Ottawa, ON, K1S 5B6, Canada

    Jorn C. C. Yu & Edward P. C. Lai

Authors
  1. Jorn C. C. Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Edward P. C. Lai
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Edward P. C. Lai.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yu, J.C.C., Lai, E.P.C. Polypyrrole modified stainless steel frits for on-line micro solid phase extraction of ochratoxin A. Anal Bioanal Chem 381, 948–952 (2005). https://doi.org/10.1007/s00216-004-2994-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-004-2994-2

Keywords

Search

Navigation