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Analysis of wheat extracts for ochratoxin A by molecularly imprinted solid-phase extraction and pulsed elution

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Abstract

A molecularly imprinted solid-phase extraction (MISPE) method has been developed for the rapid analysis of wheat extracts for ochratoxin A (OTA). Molecularly imprinted polymer (MIP) particles were synthesized from N-phenylacrylamide (PAM) and slurry-packed into a micro-column for selective solid-phase extraction (SPE) of OTA. With water flowing at 0.5 mL min−1, a total binding capacity of 30 ng OTA was determined for the 20 mg of MIP particles. MISPE conditions were optimized using OTA in methanol/acetic acid (99:1 v/v). Nearly 100% binding could be achieved from one 20-μL injection of sample containing up to 30 ng of OTA. Pulsed elution (PE) using methanol/triethylamine (99:1 v/v) was good for the quantitative desorption of OTA. The MISPE–PE method, with fluorescence detection at λ ex=385 nm and λ em=445 nm, afforded a detection limit of 5.0 ng mL−1 (or 0.1 ng in 20 μL of sample injected) for OTA. Recovery of OTA from wheat extracts was 103±3%. Each MISPE–PE analysis required less than 5 min to complete.

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References

  1. http://www.lfra.co.uk/eman/fsheet2_1.htm. Cited 1 Sept 2003

  2. OAC (1990) Ochratoxin A in green coffee 975.38

  3. Sibanda L, De Saeger S, Van Peteghem C (2002) J Chromatogr A 959:327

    Article  CAS  PubMed  Google Scholar 

  4. Stevenson D (2000) J Chromatogr B 745:39

    Article  CAS  Google Scholar 

  5. Patel PD (2002) TrAC 21:96–115

    Article  CAS  Google Scholar 

  6. vanderGaag B, Spath S, Dietrich H, Stigter E, Boonzaaijer G, van Osenbruggen T, Koopal K (2003) Food Control 14:251–254

    Google Scholar 

  7. Inoue K (2000) Prog Polym Sci 25:453

    Article  CAS  Google Scholar 

  8. Shea KJ, Yan M, Roberts MJ (eds) (2002) Molecularly imprinted materials—sensors and other devices. Materials Research Society, Warrendale, PA

  9. Wulff G, Sarhan A, Zabrocki K (1973) Tetrahedron Lett 44:4329–4332

    Article  Google Scholar 

  10. Norrlow O, Glad M, Mosbach K (1984) J Chromatogr 299:29

    Article  Google Scholar 

  11. Mullett WM, Lai EPC (1998) Anal Chem 70:3636–3641

    Google Scholar 

  12. Mullett WM, Lai EPC (1999) Microchem J 61:143–55

    Article  CAS  Google Scholar 

  13. Mullett WM, Lai EPC, Sellergren B (1999) Anal Commun 36:217–220

    Article  CAS  Google Scholar 

  14. Mullett WM, Lai EPC (1999) J Pharm Biomed Anal 21:835–843

    Article  CAS  Google Scholar 

  15. Mullet WM, Dirie MF, Lai EPC (2000) Anal Chim Acta 414:123–131

    Article  CAS  Google Scholar 

  16. Mullett WM, Martin P, Pawliszyn J (2001) Anal Chem 73:2383

    CAS  PubMed  Google Scholar 

  17. Lai EPC, Wu SG (2003) Anal Chim Acta 481:165–167

    Article  CAS  Google Scholar 

  18. Wu SG, Lai EPC, Mayer PM (2003) J Pharm Biomed Anal (in press)

  19. Lai EPC, Feng SY (2003) Microchem J 75:159–168

    Article  CAS  Google Scholar 

  20. Baggiani C, Giraudi G, Vanni A (2001) Bioseparation 10:389–394

    Article  CAS  PubMed  Google Scholar 

  21. Jodlbauer J, Maier NM, Lindner W (2002) J Chromatogr A 945:45–63

    Article  CAS  PubMed  Google Scholar 

  22. Zhou SN, Lai EPC (2003) N-Phenylacrylamide functional polymer with high affinity for ochratoxin A. React Funct Polym (submitted)

  23. Valenta H (1998) J Chromatogr A 815:75

    Article  CAS  PubMed  Google Scholar 

  24. Russel H (1986) Ruckstandsanalytic von wirkstoffen in tierischen produkten. Georg Tieme Verlag, Stuttgart

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Acknowledgments

Financial support of the Natural Sciences and Engineering Research Council (NSERC) Canada is gratefully acknowledged. The authors thank Tom Nowicki and Mike Roscoe, Winnipeg Food Inspection Agency, for the wheat extracts and helpful discussions.

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Authors and Affiliations

  1. Ottawa-Carleton Chemistry Institute, Department of Chemistry, Carleton University, Ottawa, ON K1S 5B6, Canada

    Simon N. Zhou, Edward P. C. Lai & J. David Miller

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  1. Simon N. Zhou
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  2. Edward P. C. Lai
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  3. J. David Miller
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Correspondence to Edward P. C. Lai.

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Zhou, S.N., Lai, E.P.C. & Miller, J.D. Analysis of wheat extracts for ochratoxin A by molecularly imprinted solid-phase extraction and pulsed elution. Anal Bioanal Chem 378, 1903–1906 (2004). https://doi.org/10.1007/s00216-003-2409-9

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  • DOI: https://doi.org/10.1007/s00216-003-2409-9

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