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Comparison of the analysis of corticosteroids using different techniques

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Abstract

In this work we have optimized the analysis of 18 human corticosteroids, some endogenous (tetrahydrocortisol, tetrahydrocortisone, cortisol, and cortisone) and others synthetic (betamethasone, budesonide, cortisone acetate, desonide, dexamethasone, dexamethasone acetate, flunisolide, fluocinolone acetonide, halcinonide, methylprednisolone, prednisolone, prednisone, triamcinolone, and triamcinolone acetonide). Three analytical techniques were developed: ELISA, gas chromatography coupled with mass spectrometry (GC–MS), and liquid chromatography coupled with mass spectrometry (LC–MS). Several sample-preparation methods were optimized for each technique and enabled compounds of interest to be extracted from small urine samples (several mL). The results enabled us to assess the possibilities and the sensitivity of each technique for application to doping tests.

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References

  1. Llorca G (1996) Corticostéroïdes. La revue du praticien 46

    CAS  Google Scholar 

  2. Shimada K, Mitamura K, Higashi T (2001) J Chromatogr A 935:141–172

    Article  CAS  PubMed  Google Scholar 

  3. Vine JH, Batty DC, Mason KE (1992) In: Proceedings of the 9th conference of racing analysts and veterinarians 103

  4. Yap BK, Johnston GAR, Kazlauskas R (1992) J Chromatogr Biomed Appl 573:183–190

    Article  CAS  Google Scholar 

  5. May 2001 report, Research contract No. 711972, Conseil de prévention et de lutte contre le dopage (Council for the prevention and battle against doping)

  6. Bourgogne E, Herrou V, Mathurin JC, Becchi M, De Ceaurriz J (2000) Rapid Common in Mass Spectrum 14:2343–2347

    Article  CAS  Google Scholar 

  7. Segura J, Ventura R, Jurado C (1998) J Chromatogr B 713:61–90

    CAS  Google Scholar 

  8. Fluri K, Rivier L, Dienes-Nagy A, You C, Maître A, Schweizer C, Saugy M, Mangin P (2001) J Chromatogr A 926:87–95

    Article  CAS  PubMed  Google Scholar 

  9. Volin P (1995) J Chromatogr B 671:319–340

    CAS  Google Scholar 

  10. Stouten P, Haasnoot W, Cazemier G, Berende PLM, Keukens H (1996) In: Haagsma N, Ruiter A (eds) Proceedings Euro-Residue III. p 902

  11. Courtheyn D, Verheye N, Bakeroot V, Dal V, Schilt R, Hooijerink H, van Bennekom EO, Haasnoot W, Stouten P, Huf FA (1993) Proc Euro Residue II 1:262

    Google Scholar 

  12. Brunn H, Georgii S (1994) Archiv für Lebensmittelhygiene 45(4):96

    Google Scholar 

  13. Pujos E, Flament-Waton MM, Goetinck P, Grenier-Loustalot MF (2004) Anal Bioanal Chem (accepted 4 July 2004)

    Google Scholar 

  14. Rivero-Marabé JJ, Maynar-Marino JI, Garcia-de-Tiedra MP, Galan-Martin AM, Caballero-Loscos MJ, Maynar-Marino M (2001) J Chromatogr B 761:77–84

    Google Scholar 

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Acknowledgements

We thank Dr F. Wright of the Medical Biochemistry Department of the Pitié Salpêtrière Hospital in Paris for providing urine samples from patients treated with hydrocortisone.

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  1. Service Central d’Analyses, USR 059, CNRS, BP 22, Vernaison 69390, France

    E. Pujos, M. M. Flament-Waton, O. Paisse & M. F. Grenier-Loustalot

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  1. E. Pujos
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  2. M. M. Flament-Waton
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  3. O. Paisse
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  4. M. F. Grenier-Loustalot
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Correspondence to M. F. Grenier-Loustalot.

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Pujos, E., Flament-Waton, M.M., Paisse, O. et al. Comparison of the analysis of corticosteroids using different techniques. Anal Bioanal Chem 381, 244–254 (2005). https://doi.org/10.1007/s00216-004-2890-9

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  • DOI: https://doi.org/10.1007/s00216-004-2890-9

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