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Involvement of sensory nerves and TRPV1 receptors in the rat airway inflammatory response to two environment pollutants: diesel exhaust particles (DEP) and 1,2-naphthoquinone (1,2-NQ)

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An Erratum to this article was published on 29 December 2009

Abstract

The environmental chemical 1,2-naphthoquinone (1,2-NQ) is implicated in the exacerbation of airways diseases induced by exposure to diesel exhaust particles (DEP), which involves a neurogenic-mediated mechanism. Plasma extravasation in trachea, main bronchus and lung was measured as the local 125I-bovine albumin accumulation. RT-PCR quantification of TRPV1 and tachykinin (NK1 and NK2) receptor gene expression were investigated in main bronchus. Intratracheal injection of DEP (1 and 5 mg/kg) or 1,2-NQ (35 and 100 nmol/kg) caused oedema in trachea and bronchus. 1,2-NQ markedly increased the DEP-induced responses in the rat airways in an additive rather than synergistic manner. This effect that was significantly reduced by L-732,138, an NK1 receptor antagonist, and in a lesser extent by SR48968, an NK2 antagonist. Neonatal capsaicin treatment also markedly reduced DEP and 1,2-NQ-induced oedema. Exposure to pollutants increased the TRPV1, NK1 and NK2 receptors gene expression in bronchus, an effect was partially suppressed by capsaicin treatment. In conclusion, our results are consistent with the hypothesis that DEP-induced airways oedema is highly influenced by increased ambient levels of 1,2-NQ and takes place by neurogenic mechanisms involving up-regulation of TRPV1 and tachykinin receptors.

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Acknowledgments

We thank Dra Simone A. Teixeira, Dra. Ana A. Varriano, Maria A. Barreto, Dr Enilton A. Camargo for technical support. This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP). TELES A.M. and S.K.P.C. are supported by research fellowships from CNPq.

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The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. Department of Pharmacology, Institute of Biosciences (ICB-I), University of São Paulo (USP), São Paulo, SP, 05508-900, Brazil

    Aila Mirtes Teles, Simone A. Teixeira, Ana A. Varriano, Maria Alice A. G. Barreto, Wothan Tavares de Lima, Marcelo N. Muscará & Soraia K. P. Costa

  2. Doctoral Programs in Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8575, Japan

    Yoshito Kumagai

  3. Cardiovascular Division, King’s College, New Hunt’s House, Guy’s Campus, London, UK

    Susan D. Brain

  4. Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, SP, 13083-970, Brazil

    Edson Antunes

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  1. Aila Mirtes Teles
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An erratum to this article can be found at http://dx.doi.org/10.1007/s00204-009-0506-z

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Teles, A.M., Kumagai, Y., Brain, S.D. et al. Involvement of sensory nerves and TRPV1 receptors in the rat airway inflammatory response to two environment pollutants: diesel exhaust particles (DEP) and 1,2-naphthoquinone (1,2-NQ). Arch Toxicol 84, 109–117 (2010). https://doi.org/10.1007/s00204-009-0427-x

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