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Analysis of nonribosomal peptide synthetase genes in haemolymph microbes of marine crabs

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Abstract

Nonribosomal peptide synthetases (NRPS) are multi-domain enzymes that have innumerably beneficial health applications. Realizing the significance of marine microorganisms in search for NRPS sequences, study was conducted for analysis of NRPS gene sequences of marine crab haemolymph bacteria for the first time. Strains belonging to five different species were found to have NRPS genes. The study generated NRPS sequences from four bacterial species, for which NRPS gene information was not available earlier. Two new putative adenylation domain signatures were identified from phylum Firmicutes. In silico analysis of amino acid sequences from four species showed less identity (42–50%) to the characterized NRPS compounds that integrate serine residue in active site, suggesting the novelty or uncharacterized nature. Altogether, the study warrants future research exploiting marine crab haemolymph bacteria, an unexplored niche of microbial genetic wealth to discover microbial novel NRPS genes and natural products using emerging tools and technologies.

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Data availability

The authors confirm that the data supporting the findings of this study are available within the article [and/or] its supplementary materials.

Code availability

The sequence data that generated out of the study are submitted in GenBank, NCBI and got assigned with accession codes (MN372397-MN372401). The NRPS sequences of the study were analyzed for the presence of NRPS ‘A’ domains using domain search program in the website namely, https://www.nii.ac.in/~zeeshan/search_only_nrps.html which is openly available.

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Acknowledgements

The authors are grateful to the Head, Marine Biotechnology Division and the Director, ICAR-Central Marine Fisheries Research Institute, Kochi for providing necessary facilities to carry out the present investigation. A.P.V acknowledges CSIR-UGC for the research fellowship. This work was supported by ICAR-CMFRI funded project “Health Management in selected finfish and shellfish and bio-prospecting from marine resources” (MBT/HLT/23) and research contingency amount for BSc–MSc Integrated Biotechnology Course students by Kerala Agricultural University, Thrissur to A.M.B.

Funding

This work was supported by ICAR-CMFRI-funded project “Health Management in selected finfish and shellfish and bio-prospecting from marine resources” (MBT/HLT/23) and research contingency amount for BSc–MSc Integrated Biotechnology Course students by Kerala Agricultural University, Thrissur to A.M.B.

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Author notes

  1. Sumithra Thangalazhy Gopakumar

    Present address: Marine Biotechnology Division, ICAR-Central Marine Fisheries Research Institute, Post Box No. 1603, Ernakulam North P.O., Kochi, 682 018, India

Authors and Affiliations

  1. Department of Plant Biotechnology, College of Agriculture, Vellayani, India

    Akhitha Mary Benny, Neethu Bindu Raj & Soni Kallamparambil Balakrishnan

  2. Marine Biotechnology Division, ICAR-Central Marine Fisheries Research Institute, Post Box No. 1603, Ernakulam North P.O., Kochi, 682 018, India

    Reshma Kalarical Janardhanan, Anusree Velappan Nair, Amala Panaparambil Vakkachan & Sanil Nandiath Karayi

  3. Crustacean Fisheries Division, ICAR-Central Marine Fisheries Research Institute, Post Box No. 1603, Ernakulam North P.O., Kochi, 682 018, India

    Ratheesh Kumar Raveendran

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  1. Akhitha Mary Benny
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Contributions

STG and RKJ conceived of the presented idea, supervised the findings and wrote the manuscript. AMB executed the experimental design and combined the observations. AVN, NBR, and APV provided technical support to carry out experiments. NKS supervised the project. RKR helped in the sample collection and analysis of the results. SKB provided critical feedback in drafting the manuscript.

Corresponding author

Correspondence to Sumithra Thangalazhy Gopakumar.

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Communicated by Erko Stackebrandt.

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Benny, A.M., Gopakumar, S.T., Janardhanan, R.K. et al. Analysis of nonribosomal peptide synthetase genes in haemolymph microbes of marine crabs. Arch Microbiol 203, 1251–1258 (2021). https://doi.org/10.1007/s00203-020-02101-9

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  • DOI: https://doi.org/10.1007/s00203-020-02101-9

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