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The Screening of Antimicrobial Bacteria with Diverse Novel Nonribosomal Peptide Synthetase (NRPS) Genes from South China Sea Sponges

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An Erratum to this article was published on 10 February 2009

Abstract

Nonribosomal peptide synthetase (NRPS) adenylation (A) domain genes were investigated by polymerase chain reaction for 109 bacteria isolated from four South China Sea sponges, Stelletta tenuis, Halichondria rugosa, Dysidea avara, and Craniella australiensis. Meanwhile, the antimicrobial bioassay of bacteria with NRPS genes were carried out to confirm the screening of NRPS genes. Fifteen bacteria were found to contain NRPS genes and grouped into two phyla Firmicutes (13 of 15) and Proteobacteria (two of 15) according to 16S rDNA sequences. Based on the phylogenetic analysis of the conserved A domain amino acid sequences, most of the NRPS fragments (11 of 15) showed below 70% similarity to their closest relatives suggesting the novelty of these NRPS genes. All of the 15 bacteria with NRPS genes have antimicrobial activities, with most of them exhibiting activity against multiple indicators including fungi and gram-positive and gram-negative bacteria. The different antimicrobial spectra indicate the chemical diversity of biologically active metabolites of sponge-associated bacteria and the possible role of bacterial symbionts in the host’s antimicrobial chemical defense. Phylogenetic analysis based on the representative NRPS genes shows high diversity of marine NRPS genes. The combined molecular technique and bioassay strategy will be useful to obtain sponge-associated bacteria with the potential to synthesize bioactive compounds.

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Acknowledgments

This research was supported by the “High-Tech Research and Development Program of China” (2007AA09Z447) and “Program for New Century Excellent Talents in University (NCET), China”.

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Authors and Affiliations

  1. Laboratory of Marine Biotechnology and Key Laboratory of Microbial Metabolism, Ministry of Education, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China

    Wei Zhang, Zhiyong Li, Xiaoling Miao & Fengli Zhang

  2. School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, People’s Republic of China

    Zhiyong Li

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  1. Wei Zhang
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  2. Zhiyong Li
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  3. Xiaoling Miao
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  4. Fengli Zhang
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Correspondence to Zhiyong Li.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s10126-009-9183-4

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Zhang, W., Li, Z., Miao, X. et al. The Screening of Antimicrobial Bacteria with Diverse Novel Nonribosomal Peptide Synthetase (NRPS) Genes from South China Sea Sponges. Mar Biotechnol 11, 346–355 (2009). https://doi.org/10.1007/s10126-008-9148-z

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