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Euonymus japonicus phyllosphere microbiome is significantly changed by powdery mildew

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Abstract

Euonymus japonicus Thunb. is a woody and ornamental plant popular in China, Europe and North America. Powdery mildew is one of the most serious diseases that affect E. japonicus growth. In this study, the diseased and apparently healthy leaves were collected from E. japonicus planted in a greenbelt in Beijing, and the effect of powdery mildew on the epiphytic microbial community was investigated by using Illumina sequencing. The results showed that the healthy leaves (HL) harbored greater bacterial and fungal diversity than diseased leaves (DL). Furthermore, both bacterial and fungal communities in DL exhibited significantly different structures from those in HL. The relative abundance of several bacterial phyla (Proteobacteria and Firmicutes) and fungal phyla (Ascomycota and Basidiomycota) were altered by powdery mildew. At the genus level, most genera decreased as powdery mildew pathogen Erysiphe increased, while the genera Kocuria and Exiguobacterium markedly increased. Leaf properties, especially protein content was found to significantly affect beta-diversity of the bacterial and fungal community. Network analysis revealed that positive bacterial interactions in DL were stronger than those in HL samples. Insights into the underlying the indigenous microbial phyllosphere populations of E. japonicus response to powdery mildew will help in the development of methods for controlling plant diseases.

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Acknowledgements

This study was financially supported by the National Key Research and Development Program of China (No. 2017YFD0800102), the National Natural Science Foundation of China (Nos. 31500083 and 31601642) and the Innovation Platform and Talent Plan (2016RS2019).

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Authors and Affiliations

  1. College of Plant Protection, Hunan Agricultural University, Changsha, 410128, China

    Zhuo Zhang & Pei Wang

  2. Key Laboratory of Pest Management of Horticultural Crops of Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural Science, Changsha, 410125, China

    Zhuo Zhang & Pei Wang

  3. Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China

    Xiao Kong, Decai Jin, Hao Yu & Ye Deng

  4. State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

    Xun Zhu

  5. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Xiaofeng Su

  6. Federal Institute for Geosciences and Natural Resources (BGR), Hannover, 30655, Germany

    Ruiyong Zhang

  7. Beijing Key Laboratory of Detection and Control of Spoilage Organisms and Pesticide Residues in Agricultural Products, Beijing University of Agriculture, Beijing, 102206, China

    Minghong Jia

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  1. Zhuo Zhang
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Correspondence to Decai Jin.

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Zhang, Z., Kong, X., Jin, D. et al. Euonymus japonicus phyllosphere microbiome is significantly changed by powdery mildew. Arch Microbiol 201, 1099–1109 (2019). https://doi.org/10.1007/s00203-019-01683-3

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