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Chromocurvus halotolerans gen. nov., sp. nov., a gammaproteobacterial obligately aerobic anoxygenic phototroph, isolated from a Canadian hypersaline spring

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Abstract

A strain EG19T of aerobic bacteria able to form pleomorphic cells was isolated from a brine spring runoff stream in the west central region of the province of Manitoba, Canada. The pale pinkish purple strain contained bacteriochlorophyll a incorporated into light-harvesting I and reaction center complexes. Its inability to grow under anaerobic illuminated conditions prompted designation as a member of the functional group known as aerobic anoxygenic phototrophic bacteria. Phylogenetic analysis of the 16S rRNA gene sequence revealed that it belonged to the Gammaproteobacteria, forming a distinct branch of phototrophs distantly related to most described aerobic anoxygenic phototrophs, quite marginally related (95.6%) both to the only other described gammaproteobacterial aerobic phototroph, Congregibacter litoralis, and also to nonphototrophs in the genus Haliea (95.1–96.1%). Physiological tests demonstrated tolerance profiles to salinity (0–18% NaCl), pH (7–12), and temperature (7–40°C) consistent with survival in a shallow hypersaline stream on the exposed, vegetation-depleted salt playa of its native East German Creek. Phylogenetic data and phenotypic properties such as pigment composition, morphology, and physiology support the proposal of the novel genus and species Chromocurvus halotolerans gen. nov., sp. nov., with EG19T (=DSM 23344T, =VKM B-2659T) as the type strain.

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Acknowledgments

This research was supported by grants from the Natural Science and Engineering Research Council (NSERC) (Canada) to V. Y.

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Authors and Affiliations

  1. Department of Microbiology, University of Manitoba, 418 Buller Building, Winnipeg, MB, R3T 2N2, Canada

    J. T. Csotonyi & V. Yurkov

  2. DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstrasse 7B, 38124, Braunschweig, Germany

    E. Stackebrandt, J. Swiderski & P. Schumann

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  1. J. T. Csotonyi
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  2. E. Stackebrandt
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  3. J. Swiderski
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  4. P. Schumann
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  5. V. Yurkov
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Correspondence to V. Yurkov.

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Communicated by Harals Huber.

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Csotonyi, J.T., Stackebrandt, E., Swiderski, J. et al. Chromocurvus halotolerans gen. nov., sp. nov., a gammaproteobacterial obligately aerobic anoxygenic phototroph, isolated from a Canadian hypersaline spring. Arch Microbiol 193, 573–582 (2011). https://doi.org/10.1007/s00203-011-0698-5

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  • DOI: https://doi.org/10.1007/s00203-011-0698-5

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