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Differential effects of coinoculations with Pseudomonas jessenii PS06 (a phosphate-solubilizing bacterium) and Mesorhizobium ciceri C-2/2 strains on the growth and seed yield of chickpea under greenhouse and field conditions

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Abstract

In the course of a project carried out in two regions of Spain, Castilla y León and Andalucía, aiming to find useful biofertilizers for staple grain-legumes, an efficient rhizobia nodulating chickpea (termed as C-2/2) and a powerful in vitro phosphate-solubilizing bacterial strain (termed as PS06) were isolated. Analyses of their 16S rDNA sequence indicated that they belong to the bacterial species Mesorhizobium ciceri and Pseudomonas jessenii, respectively. Greenhouse and field experiments were carried out in order to test the effect of single and dual inoculations on chickpea (ecotype ILC-482) growth. Under greenhouse conditions, plants inoculated with Mesorhizobium ciceri C-2/2 alone had the highest shoot dry weight. The inoculation treatment with P. jessenii PS06 yielded a shoot dry weight 14% greater than the uninoculated control treatment, but it was not correlated with shoot P contents. However, the co-inoculation of C-2/2 with PS06 resulted in a decrease in shoot dry weight with respect to the inoculation with C-2/2 alone. Under field conditions, plants inoculated with M. ciceri C-2/2, in single or dual inoculation, produced higher nodule fresh weight, nodule number and shoot N content than the other treatments. Inoculation with P. jessenii PS06 had no significant effect on plant growth. However, the co-inoculation treatment ranked the highest in seed yield (52% greater than the uninoculated control treatment) and nodule fresh weight. These data suggest that P. jessenii PS06 can act synergistically with M. ciceri C-2/2 in promoting chickpea growth. The contrasting results obtained between greenhouse and field experiments are discussed.

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Acknowledgements

This work was supported by the Junta de Castilla y León and the Junta de Andalucía. The authors thank the soil analyses service staff from IRNA for their help in this work.

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Authors and Affiliations

  1. Instituto de Recursos Naturales y Agrobiología-CSIC, Apartado 257, 37071, Salamanca, Spain

    Angel Valverde, Tiziana Fiscella, Claudino Rodríguez-Barrueco, Emilio Cervantes & José-Mariano Igual

  2. CIFA Las Torres-Tomejil, 41200, Alcalá del Rio, Sevilla, Spain

    Araceli Burgos & Manuel Chamber

  3. Departamento de Microbiología y Genética, Universidad de Salamanca, 37007, Salamanca, Spain

    Raúl Rivas & Encarna Velázquez

  4. Agriculture Faculty, University of Catania, Via Valdisaboia 5, 95123, Catania, Italy

    Tiziana Fiscella

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  1. Angel Valverde
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Correspondence to José-Mariano Igual.

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Valverde, A., Burgos, A., Fiscella, T. et al. Differential effects of coinoculations with Pseudomonas jessenii PS06 (a phosphate-solubilizing bacterium) and Mesorhizobium ciceri C-2/2 strains on the growth and seed yield of chickpea under greenhouse and field conditions. Plant Soil 287, 43–50 (2006). https://doi.org/10.1007/s11104-006-9057-8

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