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Characterization of a novel T4-type Stenotrophomonas maltophilia virulent phage Smp14

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Abstract

Stenotrophomonas maltophilia (Sm), with most of the isolates being resistant to multidrugs, is an opportunistic bacterium causing nosocomial infections. In this study, a novel virulent Sm phage, Smp14, was characterized. Electron microscopy showed that Smp14 resembled members of Myoviridae and adsorbed to poles of the host cells during infection. It lysed 37 of 87 clinical Sm isolates in spot test, displayed a latent period of ca. 20 min, and had a burst size of ca. 150. Its genome (estimated to be 160 kb by PFGE), containing m4C and two unknown modified bases other than m5C and m6A as identified by HPLC, resisted to digestion with many restriction endonucleases except MseI. These properties indicate that it is a novel Sm phage distinct from the previously reported phiSMA5 which has a genome of 250 kb digestible with various restriction enzymes. Sequencing of a 16 kb region revealed 12 ORFs encoding structural proteins sharing 15–45% identities with the homologues from T4-type phages. SDS-PAGE displayed 20 virion proteins, with the most abundant one being the 39 kDa major capsid protein (gp23), which had the N-terminal 52 amino acids removed. Phylogenetic analysis based on gp23 classified Smp14 into a novel single-membered T4-type subgroup.

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Authors and Affiliations

  1. Institute of Molecular Biology, National Chung Hsing University, Taichung, 402, Taiwan

    Chiy-Rong Chen, Ching-Hsuan Lin & Shu-Fen Weng

  2. Institute of Biochemistry, National Chung Hsing University, Taichung, 402, Taiwan

    Juey-Wen Lin

  3. Graduate Institute of Biotechnology, National Pingtung University of Science and Technology, Pingtung, 912, Taiwan

    Chi-I Chang

  4. Institute of Medical Biotechnology, Central Taiwan University of Science and Technology, Taichung, 406, Taiwan

    Yi-Hsiung Tseng

Authors
  1. Chiy-Rong Chen
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  2. Ching-Hsuan Lin
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  3. Juey-Wen Lin
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  4. Chi-I Chang
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  5. Yi-Hsiung Tseng
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  6. Shu-Fen Weng
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Corresponding authors

Correspondence to Yi-Hsiung Tseng or Shu-Fen Weng.

Additional information

Communicated by Jorge Membrillo-Hernández.

Chiy-Rong Chen and Ching-Hsuan Lin contributed equally.

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Chen, CR., Lin, CH., Lin, JW. et al. Characterization of a novel T4-type Stenotrophomonas maltophilia virulent phage Smp14. Arch Microbiol 188, 191–197 (2007). https://doi.org/10.1007/s00203-007-0238-5

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  • DOI: https://doi.org/10.1007/s00203-007-0238-5

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