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The effects of collector geometry on the internal structure of the 3D nanofiber scaffold fabricated by divergent electrospinning

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Abstract

Latest bioinspired approaches in tissue engineering focus on the creation of biomaterials with micro- and nanoscale topographical features. Various additive manufacturing techniques have been applied for scaffold fabrication; however, creating three dimensional (3D) nanofiber structure within a scaffold remains to be challenging. This paper presented an innovative divergence electrospinning strategy to fabricate 3D polycaprolactone (PCL) scaffolds comprised of uniaxially aligned nanofibers. The effects of collector geometry on the nanofiber structure were characterized by polynomial regression analysis. The length-to-width ratio and inclination angle of the collector were found to be critical to nanofiber distribution within the 3D scaffold. The nanofiber orientation was consistent with the direction of electric field vectors between the two bevels of the collector. After a continuous culturing for 7 days, fibroblast cells were uniaxially organized within the 3D scaffolds, closely resembling the fibrous structure in musculoskeletal tissues. This study provided a novel approach to biomimetic native tissue microstructures and showed a great potential as a future fabrication additive manufacturing platform for tissue engineering.

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Funding

This work was financially supported by the Foundation of the Whitacre College of Engineering and the Office of Vice President for Research at Texas Tech University.

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Authors and Affiliations

  1. Department of Industrial Manufacturing & Systems Engineering, Texas Tech University, Lubbock, TX, 79409, USA

    Yingge Zhou, Zhiyong Hu, Dongping Du & George Z. Tan

Authors
  1. Yingge Zhou
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  2. Zhiyong Hu
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  3. Dongping Du
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  4. George Z. Tan
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Correspondence to George Z. Tan.

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Zhou, Y., Hu, Z., Du, D. et al. The effects of collector geometry on the internal structure of the 3D nanofiber scaffold fabricated by divergent electrospinning. Int J Adv Manuf Technol 100, 3045–3054 (2019). https://doi.org/10.1007/s00170-018-2899-4

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  • DOI: https://doi.org/10.1007/s00170-018-2899-4

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