Abstract
This study presents a one-station 3D fabrication technique of nanofibrous scaffold for tissue engineering. A divergence static electric field was introduced in an electrospinning system to induce a self-assembly of aligned polycaprolactone (PCL) nanofibers into a tunable 3D architecture with thickness ranging from 2 to 12 mm. Silver nanoparticles were incorporated into the PCL solution to alter the electrical conductivity. Human fibroblast cells were cultured on the pure PCL nanofiber scaffolds in vitro for 7 days. It was found that the occurrence of nanofiber bridging phenomenon depended on the solution viscosity. The minimum viscosity to form a 3D nanofiber structure was higher than that to form a 2D nanofiber mat. The homogeneity of nanofiber distribution within the 3D space was positively correlated with the electrical conductivity and the weight of the nanofibers. In the cell culture test, fibroblasts proliferated on the scaffold and organized as an aligned matrix which mimicked the microstructure of native musculoskeletal tissues.
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M. Santoro, S.R. Shah, J.L. Walker, and A.G. Mikos, Adv. Drug Deliv. Rev. 107, 206 (2016).
C.J. Connon, Procedia Eng. 110, 15 (2015).
B.M. Holzapfel, F. Wagner, L. Thibaudeau, J.-P. Levesque, and D.M. Hutmacher, Stem Cells 33, 1696 (2015).
A. Skardal, M. Devarasetty, H.-W. Kang, I. Mead, C. Bishop, T. Shupe, S.J. Lee, J. Jackson, J. Yoo, S. Soker, and A. Atala, Acta Biomater. 25, 24 (2015).
P.M. Mendes, Chem. Soc. Rev. 42, 9207 (2013).
B.M. Baker and C.S. Chen, J. Cell Sci. 125, 3015 (2012).
H.P. Shih, D. Panlasigui, V. Cirulli, and M. Sander, Cell Rep. 14, 169 (2016).
C.S. Chen, J. Cell Sci. 121, 3285 (2008).
Y. Zhou and G.Z. Tan, Nanomater. Nanotechnol. 7, 1847980417748478 (2017).
A. Greiner and J.H. Wendorff, Angew. Chem. Int. Ed. 46, 5670 (2007).
X. Wang, B. Ding, and B. Li, Mater. Today 16, 229 (2013).
F. Du, H. Wang, W. Zhao, D. Li, D. Kong, J. Yang, and Y.Y. Zhang, Biomaterials 33, 762 (2012).
M. Ramalingam, M.F. Young, V. Thomas, L. Sun, L.C. Chow, C.K. Tison, K. Chatterjee, W.C. Miles, and C.G. Simon Jr, J. Biomater. Appl. 27, 695 (2013).
Y.J. Son, W.J. Kim, and H.S. Yoo, Arch. Pharm. Res. 37, 69 (2014).
S.B. Orr, A. Chainani, K.J. Hippensteel, A. Kishan, C.L. Gilchrist, N.W. Garrigues, D.S. Ruch, F. Guilak, and D.W. Little, Acta Biomater. 24, 117 (2015).
D. Kai, M.P. Prabhakaran, B. Stahl, M. Eblenkamp, E. Wintermantek, and S. Ramakrishna, Nanotechnology 23, 095705 (2012).
M. Deng, S.G. Kumbar, L.S. Nair, A.L. Weikel, H.R. Allcock, and C.T. Laurencin, Adv. Funct. Mater. 21, 2641 (2011).
B. Wulkersdorfer, K.K. Kao, V.G. Agopian, A. Ahn, J.C. Dunn, B.M. Wu, and M. Stelzner, Int. J. Polym. Sci. (2010). https://doi.org/10.1155/2010/436178.
B. Ostrowska, J. Jaroszewicz, E. Zaczynska, W. Tomaszewski, W. Swieszkowski, and K.J. Kurzydlowsk, Bull. Pol. Acad. Sci. Tech. Sci. 62, 551 (2014).
Y. Zhou and G.Z. Tan, Generation of 3D nanofiber structure by divergence electrospinning for tissue engineering scaffold, in ASME 2018 13th International Manufacturing Science and Engineering Conference. 2018. American Society of Mechanical Engineers, Paper No. MSEC2018-6543
B.E. Mbondo Tsamba, S. Sarraute, M. Traïkia, and P. Husson, J. Chem. Eng. Data 59, 1747 (2014).
P. Gupta, C. Elkins, T.E. Long, and G.L. Wilkes, Polymer 46, 4799 (2005).
S. Huan, G.X. Liu, G.G. Han, W.L. Cheng, Z.Y. Fu, Q.L. Wu, and Q.W. Wang, Materials 8, 2718 (2015).
A. Balogh, B. Farkas, A. Pálvölgyi, A. Domokos, B. Démuth, G. Marosi, and Z.K. Nagy, J. Pharm. Sci. 106, 1634 (2017).
K.A.G. Katsogiannis, G.T. Vladisavljević, and S. Georgiadou, Eur. Polym. J. 69, 284 (2015).
D. Kai, M.J. Tan, M.P. Prabhakaran, B.Q.Y. Chan, S.S. Liow, S. Ramakrishna, and X.J. Loh, Colloids Surf. B 148, 557 (2016).
S. Maharubin, Y. Zhou, and G.Z. Tan, IEEE Trans. Nanotechnol. 17, 948 (2018).
Y. Zhou, S. Maharubin, P. Tran, T. Reid, and G.Z. Tan, Environ. Sci. Water Res. Technol. 4, 1511 (2018).
Z. Tan, E.A. Havell, P.E. Orndorff, and R.A. Shirwaiker, BioMetals 30, 113 (2017).
Z. Tan, G.N. Xu, P.E. Orndorff, and R.A. Shirwaiker, J. Med. Biol. Eng. 36, 325 (2016).
Y. Wang, P.F. Li, P. Xiang, J.T. Lu, J. Yuan, and J. Shen, J. Mater. Chem. B 4, 635 (2016).
Y. Liu, C. Hou, T. Jiao, J. Song, X. Zhang, R. Xing, J. Zhou, L. Zhang, and Q. Peng, Nanomaterials 8, 35 (2018).
S.Y. Chew, R. Mi, A. Hoke, and K.W. Leong, Biomaterials 29, 653 (2008).
L. He, S. Kiao, D. Quan, K. Ma, C. Chan, S. Ramakrishna, and J. Lu, Acta Biomater. 6, 2960 (2010).
T. Jiang, E.J. Carbone, K.W.-H. Lo, and C.T. Laurencin, Prog. Polym. Sci. 46, 1 (2015).
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Zhou, Y., Thakurathi, M., Quitevis, E.L. et al. Electrospinning 3D Nanofiber Structure of Polycaprolactone Incorporated with Silver Nanoparticles. JOM 71, 956–962 (2019). https://doi.org/10.1007/s11837-018-3222-4
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DOI: https://doi.org/10.1007/s11837-018-3222-4