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Mit Gentamicin oder Levofloxacin gesättigter Kollagenhydroxyapatit (Healos®)

Antimikrobielle In-vitro-Wirksamkeit – eine Pilotstudie

Collagen hydroxyapatite (Healos®) saturated with gentamicin or levofloxacin

In vitro antimicrobial effectiveness – a pilot study

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Zusammenfassung

Hintergrund

Neben der strukturgebenden Funktion können Knochenersatzstoffe auch als Vehikel für die lokale Antibiotikafreisetzung dienen, um Infektionen der Knochen vorzubeugen oder zu behandeln.

Material und Methoden

Die Sättigung und antibiotische Wirksamkeit von Gentamicin und Levofloxacin mit Healos® wurde in vitro überprüft und mit Healos® ohne Antibiotikazusatz verglichen. Die antibiotisch gesättigten Knochenersatzstoffe wurden unverdünnt und in 10- sowie 100facher Verdünnung auf ihre Aktivität gegen spondylodiszitisspezifische Keime auf verschiedenen Agarplatten nach der Agardiffusionsmethode getestet.

Ergebnisse

Alle antibiotikumgesättigten und verdünnten Kollagen/HA-Proben zeigten ellipsenförmige Hemmhöfe auf den entsprechenden Agarplatten. Für beide Antibiotika konnte ein linearer Zusammenhang zwischen Hemmhofgröße und Verdünnung festgestellt werden.

Schlussfolgerung

Die antibakterielle Wirkung des antibiotikumgesättigten Kollagen/HA entspricht der antibiotischen Verdünnung. Die Ergebnisse sollten auf In-vivo-Studien ausgeweitet werden, um die antibakterielle Effizienz der Antibiotikum gesättigten Knochenersatzstoffe nach Implantation zu bestimmen.

Abstract

Background

The functions of synthetic bone graft substitutes include not only structural support to provide bone healing and osseous ingrowth but also the ability to serve as a local antibiotic delivery system to prevent or treat infections of the spine.

Material and Methods

The impregnation and antibiotic efficiency of gentamicin and levofloxacin with Healos was investigated in vitro and compared with Healos without an antibiotic additive. These antibiotic-loaded bone graft substitutes were examined without dilution and with 10-fold and 100-fold dilution for activity against spondylodiscitis-causing bacteria on different agar plates using an agar diffusion method.

Results

All hydroxyapatite (HA)/collagen-saturated diluted antibiotics showed elliptical inhibition zones on the corresponding agar plates. For both antibiotics, there was a linear correlation between dilution and area of the inhibition zone.

Conclusion

The analysis showed that the antimicrobial activity of HA/collagen-saturated antibiotics corresponded to the antimicrobial dilutions. These results should be further analyzed using in vivo studies to determine the remaining antibiotic efficiency after implantation of bone graft substitutes.

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Interessenkonflikt

Der korrespondierende Autor weist auf folgende Beziehung hin: Der Knochenersatzstoff Healos® wurde von der Fa. DePuy in Form von Drittmitteln zur Verfügung gestellt. Trotz des möglichen Interessenkonflikts ist der Beitrag unabhängig und produktneutral.

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Authors and Affiliations

  1. Querschnittzentrum und Wirbelsäulenchirurgie, Orthopädische Universitätsklinik, Schlierbacher Landstraße 200a, 69118, Heidelberg, Deutschland

    C.H. Fürstenberg, B. Wiedenhöfer, C. Putz & K. Schröder

  2. Institut für Hygiene und Medizinische Mikrobiologie, Universität, Heidelberg, Deutschland

    I. Burckhardt

  3. Abteilung für experimentelle Orthopädie, Orthopädische Universitätsklinik, Heidelberg, Deutschland

    S. Gantz & K. Kleinschmidt

Authors
  1. C.H. Fürstenberg
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  2. B. Wiedenhöfer
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  3. C. Putz
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  4. I. Burckhardt
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  5. S. Gantz
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  6. K. Kleinschmidt
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  7. K. Schröder
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Corresponding author

Correspondence to C.H. Fürstenberg.

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Fürstenberg, C., Wiedenhöfer, B., Putz, C. et al. Mit Gentamicin oder Levofloxacin gesättigter Kollagenhydroxyapatit (Healos®). Orthopäde 39, 437–443 (2010). https://doi.org/10.1007/s00132-009-1528-1

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  • DOI: https://doi.org/10.1007/s00132-009-1528-1

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