Abstract
Key message
Combination of RL4137 alleles at three QTLs on chromosomes 4A, 6B and 6D, and ‘Roblin’ allele at a novel QTL on chromosome 1D increases pre-harvest sprouting resistance in ‘Roblin’/RL4137 doubled haploid population.
Abstract
Pre-harvest sprouting (PHS) significantly reduces wheat grain yield and quality. Therefore, identifying quantitative trait loci (QTL) for PHS resistance is key to facilitate marker-assisted breeding. To this end, we studied PHS in a population of 330 doubled haploid (DH) lines derived from ‘Roblin’/RL4137. The parental and DH lines were examined for their PHS phenotype based on speed of germination index in five environments and genotyped using the wheat Infinium 90 K SNP array. A total of five QTLs were detected on linkage groups 1D, 4A.2, 6B.1, 6D and 7A over the five environments. The QTL QPhs.umb-4A on linkage group 4A.2 was the most consistent across all environments and explained 40–50% of phenotypic variation. The QTL on 1D is a novel QTL and explained 1.99–2.33% of phenotypic variation. The QTLs on 6B.1 and 6D each explained 3.09–4.33% and 1.62–2.45% of phenotypic variation, respectively. A combination of four stable QTLs on linkage groups 1D, 4A.2, 6B.1 and 6D greatly increased PHS resistance. Allelic effects for the QTLs QPhs.umb-4A, QPhs.umb-6B and QPhs.umb-6D were contributed by RL4137, whereas ‘Roblin’ contributed the resistant allele for QPhs.umb-1D. QPhs.umb-4A was required for strong dormancy in the ‘Roblin’/RL4137 DH population, and the presence of QTLs QPhs.umb-1D, QPhs.umb-6B and QPhs.umb-6D incrementally increased dormancy; DH lines carrying all four QTLs are considerably more dormant than those carrying only QPhs.umb-4A or none of the four QTLs. Thus, the QTLs identified in this study have the potential to improve PHS resistance in spring wheat.
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Acknowledgements
The authors would like to thank Dr. Anita Brûlé-Babel’s laboratory for their support in setting up the trails at Point location and Dr. Doug Cattani’s laboratory for their assistance in threshing the experimental samples. We also thank all graduate students of Dr. Ayele’s laboratory for their help in harvesting the trials and technical staff of all the laboratories involved in this research for their technical assistance. This work was supported by a grant from the Natural Sciences and Engineering Research Council of Canada (Grant No. ALLRP 545510-19), Manitoba Crop Alliance (formerly Manitoba Wheat and Barley Growers Association), and Canadian Agricultural Partnership-Ag Action Manitoba Program to BTA. UAL is recipient of the University of Manitoba Graduate Fellowship.
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BTA and MCJ conceived, designed and supervised the research; UAL and CAM performed the experiments and analyzed the data; CWH generated the genotypic data; SK supervised the management of the field trial at Brandon; UAL wrote the first draft of the manuscript; BTA and CAM edited and revised the manuscript; and all authors read and approved the final manuscript.
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Liton, M.M.U.A., McCartney, C.A., Hiebert, C.W. et al. Identification of loci for pre-harvest sprouting resistance in the highly dormant spring wheat RL4137. Theor Appl Genet 134, 113–124 (2021). https://doi.org/10.1007/s00122-020-03685-y
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DOI: https://doi.org/10.1007/s00122-020-03685-y