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Fine mapping of the Brassica napus Bnsdt1 gene associated with determinate growth habit

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The newly discovered determinate plant growth habit of Brassica napus is a potential trait that might contribute to the genetic improvement of rapeseed.

Abstract

Brassica napus is an important species of rapeseed and has an indeterminate growth habit. However, a determinate inflorescence strain (4769) has been discovered among doubled haploid (DH) lines obtained from a spring B. napus × winter B. napus cross. We assessed the effect of the determinate growth habit on agronomic traits. The results showed that determinacy is beneficial for reducing plant height and flowering time, advancing maturity and maintaining productivity. We also investigated the inheritance of determinacy. A genetic analysis revealed that the phenotype of the determinate trait is controlled by one recessive gene, Bnsdt1. Mapping of the Bnsdt1 gene was subsequently conducted in BC1 and BC3 populations derived from combination 2014 × 4769. The results showed that the Bnsdt1 gene could be delimited to a region of approximately 220 kb, between 16,627 and 16,847 kb on A10. Within the target region, whole-genome re-sequencing identified two candidate regions (16,628–16,641 and 16,739–16,794 kb) of approximately 68 kb. A Blast analysis of the two candidate intervals found that BnaA10g26300D/GSBRNA2T00136426001 (BnTFL1) is homologous to the TFL1 gene of A. thaliana. Subsequently, quantitative reverse transcription (qRT)-PCR revealed that BnTFL1 was specifically expressed in the shoot apex. Collectively, the results of expression analysis provide preliminary evidence that BnTFL1 is a candidate gene for the inflorescence trait in 4769.

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Acknowledgements

This research was financially supported by the National System of Technology of the Rapeseed Industry (NYCYTX-00516), the National Key Research and Development Plan of China (2016YFD0100202) and Key Laboratory of Spring Rape Genetic Improvement of Qinghai Province (2017-ZJ-Y09).

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Authors and Affiliations

  1. State Key Laboratory of Plateau Ecology and Agriculture of Qinghai University, Key Laboratory of Spring Rape Genetic Improvement of Qinghai Province, Academy of Agricultural and Forestry Sciences of Qinghai University, Xining, 810016, Qinghai, China

    Kaixiang Li, Yanmei Yao, Lu Xiao, Zhigang Zhao, Shaomin Guo, Zhong Fu & Dezhi Du

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  1. Kaixiang Li
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  2. Yanmei Yao
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  3. Lu Xiao
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  4. Zhigang Zhao
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  5. Shaomin Guo
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  6. Zhong Fu
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  7. Dezhi Du
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Correspondence to Dezhi Du.

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Communicated by Maria Laura Federico.

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Li, K., Yao, Y., Xiao, L. et al. Fine mapping of the Brassica napus Bnsdt1 gene associated with determinate growth habit. Theor Appl Genet 131, 193–208 (2018). https://doi.org/10.1007/s00122-017-2996-z

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  • DOI: https://doi.org/10.1007/s00122-017-2996-z

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