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Unlocking new alleles for leaf rust resistance in the Vavilov wheat collection

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Thirteen potentially new leaf rust resistance loci were identified in a Vavilov wheat diversity panel. We demonstrated the potential of allele stacking to strengthen resistance against this important pathogen.

Abstract

Leaf rust (LR) caused by Puccinia triticina is an important disease of wheat (Triticum aestivum L.), and the deployment of genetically resistant cultivars is the most viable strategy to minimise yield losses. In this study, we evaluated a diversity panel of 295 bread wheat accessions from the N. I. Vavilov Institute of Plant Genetic Resources (St Petersburg, Russia) for LR resistance and performed genome-wide association studies (GWAS) using 10,748 polymorphic DArT-seq markers. The diversity panel was evaluated at seedling and adult plant growth stages using three P. triticina pathotypes prevalent in Australia. GWAS was applied to 11 phenotypic data sets which identified a total of 52 significant marker–trait associations representing 31 quantitative trait loci (QTL). Among them, 29 QTL were associated with adult plant resistance (APR). Of the 31 QTL, 13 were considered potentially new loci, whereas 4 co-located with previously catalogued Lr genes and 14 aligned to regions reported in other GWAS and genomic prediction studies. One seedling LR resistance QTL located on chromosome 3A showed pronounced levels of linkage disequilibrium among markers (r 2 = 0.7), suggested a high allelic fixation. Subsequent haplotype analysis for this region found seven haplotype variants, of which two were strongly associated with LR resistance at seedling stage. Similarly, analysis of an APR QTL on chromosome 7B revealed 22 variants, of which 4 were associated with resistance at the adult plant stage. Furthermore, most of the tested lines in the diversity panel carried 10 or more combined resistance-associated marker alleles, highlighting the potential of allele stacking for long-lasting resistance.

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Acknowledgements

This research was supported by an Early Career Research Grant and a Ph.D. scholarship from the University of Queensland (UQ), Australia. We thank Dr. Laura Ziems and Dr. Dharmendra Singh from UQ for their valuable advice regarding the analyses performed in this study. We also greatly appreciate valuable advice from Prof. Robert McIntosh from the University of Sydney for positioning the catalogued Lr genes in the wheat genome.

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Authors and Affiliations

  1. Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD, Australia

    Adnan Riaz, Naveenkumar Athiyannan, Sambasivam K. Periyannan, Lee T. Hickey & Kai P. Voss-Fels

  2. Commonwealth Scientific and Industrial Research Organization, Agriculture and Food, Canberra, ACT, Australia

    Naveenkumar Athiyannan, Sambasivam K. Periyannan & Evans S. Lagudah

  3. Department of Plant Resistance to Diseases, All-Russian Research Institute for Plant Protection, St Petersburg, Russia

    Olga Afanasenko

  4. N. I. Vavilov Institute of Plant Genetic Resources, St Petersburg, Russia

    Olga P. Mitrofanova

  5. Department of Agriculture and Fisheries, Hermitage Research Facility, Warwick, QLD, Australia

    Gregory J. Platz

  6. School of Agriculture and Food Sciences, The University of Queensland, St Lucia, QLD, Australia

    Elizabeth A. B. Aitken

  7. Department of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Giessen, Germany

    Rod J. Snowdon & Kai P. Voss-Fels

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  1. Adnan Riaz
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  2. Naveenkumar Athiyannan
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  3. Sambasivam K. Periyannan
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  4. Olga Afanasenko
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  5. Olga P. Mitrofanova
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  6. Gregory J. Platz
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  7. Elizabeth A. B. Aitken
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  8. Rod J. Snowdon
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  9. Evans S. Lagudah
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  10. Lee T. Hickey
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  11. Kai P. Voss-Fels
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Corresponding authors

Correspondence to Lee T. Hickey or Kai P. Voss-Fels.

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Communicated by Steven S. Xu.

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Riaz, A., Athiyannan, N., Periyannan, S.K. et al. Unlocking new alleles for leaf rust resistance in the Vavilov wheat collection. Theor Appl Genet 131, 127–144 (2018). https://doi.org/10.1007/s00122-017-2990-5

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  • DOI: https://doi.org/10.1007/s00122-017-2990-5

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