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Fine mapping of a dominantly inherited powdery mildew resistance major-effect QTL, Pm1.1, in cucumber identifies a 41.1 kb region containing two tandemly arrayed cysteine-rich receptor-like protein kinase genes

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A dominantly inherited major-effect QTL for powdery mildew resistance in cucumber was fine mapped. Two tandemly arrayed cysteine-rich receptor-like protein kinase genes were identified as the most possible candidates.

Abstract

Powdery mildew (PM) is one of the most severe fungal diseases of cucumber (Cucumis sativus L.) and other cucurbit crops, but the molecular genetic mechanisms of powdery mildew resistance in cucurbits are still poorly understood. In this study, through marker-assisted backcrossing with an elite cucumber inbred line, D8 (PM susceptible), we developed a single-segment substitution line, SSSL0.7, carrying 95 kb fragment from PM resistance donor, Jin5-508, that was defined by two microsatellite markers, SSR16472 and SSR16881. A segregating population with 3600 F2 plants was developed from the SSSL0.7 × D8 mating; segregation analysis confirmed a dominantly inherited major-effect QTL, Pm1.1 in cucumber chromosome 1 underlying PM resistance in SSSL0.7. New molecular markers were developed through exploring the next generation resequenced genomes of Jin5-508 and D8. Linkage analysis and QTL mapping in a subset of the F2 plants delimited the Pm1.1 locus into a 41.1 kb region, in which eight genes were predicted. Comparative gene expression analysis revealed that two concatenated genes, Csa1M064780 and Csa1M064790 encoding the same function of a cysteine-rich receptor-like protein kinase, were the most likely candidate genes. GFP fusion protein-aided subcellular localization indicated that both candidate genes were located in the plasma membrane, but Csa1M064780 was also found in the nucleus. This is the first report of dominantly inherited PM resistance in cucumber. Results of this study will provide new insights into understanding the phenotypic and genetic mechanisms of PM resistance in cucumber. This work should also facilitate marker-assisted selection in cucumber breeding for PM resistance.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 31171978), the National Key Basic Research Program of China (973 Program) (No. 2012CB113900), National Science & Technology Project for Rural Area (No. 2012AA100103), International Foundation for Science (No. C/5150-1) and Jiangsu Science & Technology Project (BE2012326).

Author contributions statement

X.C. and Y.W. conceived the experiment. X.X. and T.Y. performed the research. R.X., Y.S. and X.L. collected data. X.X., Y.W., Q.X. and X.Q. analyzed the data and wrote the manuscript. All authors reviewed and approved this submission.

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    Authors and Affiliations

    1. School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, Jiangsu, China

      Xuewen Xu, Ting Yu, Ruixue Xu, Yang Shi, Xiaojian Lin, Qiang Xu, Xiaohua Qi & Xuehao Chen

    2. USDA-ARS Vegetable Crops Research Unit, Horticulture Department, University of Wisconsin, Madison, WI, 53706, USA

      Yiqun Weng

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    1. Xuewen Xu
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    Communicated by M. J. Havey.

    Xuewen Xu and Ting Yu have contributed equally to this work.

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    Fig. S1

    Comparison of expression level of six predicted genes in the Pm1.1 candidate gene region (Csa1M064720.1, Csa1M064730.1, Csa1M064740.1, Csa1M064750.1, Csa1M064760.1 and Csa1M064770.1). Data are the means of three replicates (±SD). The cucumber β-actin gene was used as an internal control. Each value denotes the mean relative level of expression of three replicates. Means with the same lowercase letter do not significantly differ by the least significant difference (LSD) test at p ≤ 0.05 with a completely randomized design. (JPEG 2668 kb)

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    Xu, X., Yu, T., Xu, R. et al. Fine mapping of a dominantly inherited powdery mildew resistance major-effect QTL, Pm1.1, in cucumber identifies a 41.1 kb region containing two tandemly arrayed cysteine-rich receptor-like protein kinase genes. Theor Appl Genet 129, 507–516 (2016). https://doi.org/10.1007/s00122-015-2644-4

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