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Development and characterization of wheat-Ae. searsii Robertsonian translocations and a recombinant chromosome conferring resistance to stem rust

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Abstract

The emergence of a new highly virulent race of stem rust (Puccinia graminis tritici), Ug99, rapid evolution of new Ug99 derivative races overcoming resistance of widely deployed genes, and spread towards important wheat growing areas now potentially threaten world food security. Exploiting novel genes effective against Ug99 from wild relatives of wheat is one of the most promising strategies for the protection of the wheat crop. A new source of resistance to Ug99 was identified in the short arm of the Aegilops searsii chromosome 3Ss by screening wheat- Ae. searsii introgression libraries available as individual chromosome and chromosome arm additions to the wheat genome. For transferring this resistance gene into common wheat, we produced three double-monosomic chromosome populations (3A/3Ss, 3B/3Ss and 3D/3Ss) and then applied integrated stem rust screening, molecular maker analysis, and cytogenetic analysis to identify resistant wheat-Ae. searsii Robertsonian translocation. Three Robertsonian translocations (T3AL·3SsS, T3BL·3SsS and T3DL·3SsS) and one recombinant (T3DS-3SsS·3SsL) with stem rust resistance were identified and confirmed to be genetically compensating on the basis of genomic in situ hybridization, analysis of 3A, 3B, 3D and 3SsS-specific SSR/STS-PCR markers, and C-banding. In addition, nine SSR/STS-PCR markers of 3SsS-specific were developed for marker-assisted selection of the resistant gene. Efforts to reduce potential linkage drag associated with 3SsS of Ae. searsii are currently under way.

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Acknowledgments

This research was part of the project “Durable Rust Resistance in Wheat” supported by Bill and Melinda Gates Foundation and a special USDA-CSREES grant to the Wheat Genetic and Genomic Resources Center at Kansas State University. We thank W. John Raupp for critical editorial review of the manuscript and Shuangye Wu for her technical assistance. This is contribution number 11-148-J from the Kansas Agricultural Experiment Station, Kansas State University, Manhattan, KS 66506-5502, USA.

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Author notes

  1. Michael O. Pumphrey

    Present address: Spring Wheat Breeding and Genetics, Department of Crop and Soil Sciences, 291D Johnson Hall, Washington State University, Pullman, WA, 99164-6420, USA

Authors and Affiliations

  1. Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS, 66506-5502, USA

    Wenxuan Liu, Bernd Friebe & Bikram Gill

  2. USDA-ARS Hard Winter Wheat Genetics Research Unit, Manhattan, KS, 66506, USA

    Michael O. Pumphrey

  3. USDA-ARS Cereal Disease Laboratory, University of Minnesota, St. Paul, MN, 55108, USA

    Yue Jin & Matthew Rouse

Authors
  1. Wenxuan Liu
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  2. Yue Jin
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  3. Matthew Rouse
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  4. Bernd Friebe
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  5. Bikram Gill
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  6. Michael O. Pumphrey
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Corresponding author

Correspondence to Bernd Friebe.

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Communicated by B. Keller.

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Liu, W., Jin, Y., Rouse, M. et al. Development and characterization of wheat-Ae. searsii Robertsonian translocations and a recombinant chromosome conferring resistance to stem rust. Theor Appl Genet 122, 1537–1545 (2011). https://doi.org/10.1007/s00122-011-1553-4

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  • DOI: https://doi.org/10.1007/s00122-011-1553-4

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