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Linkage mapping and identification of QTL affecting deoxynivalenol (DON) content (Fusarium resistance) in oats (Avena sativa L.)

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Abstract

Mycotoxins caused by Fusarium spp. is a major concern on food and feed safety in oats, although Fusarium head blight (FHB) is often less apparent than in other small grain cereals. Breeding resistant cultivars is an economic and environment-friendly way to reduce toxin content, either by the identification of resistance QTL or phenotypic evaluation. Both are little explored in oats. A recombinant-inbred line population, Hurdal × Z595-7 (HZ595, with 184 lines), was used for QTL mapping and was phenotyped for 3 years. Spawn inoculation was applied and deoxynivalenol (DON) content, FHB severity, days to heading and maturity (DH and DM), and plant height (PH) were measured. The population was genotyped with DArTs, AFLPs, SSRs and selected SNPs, and a linkage map of 1,132 cM was constructed, covering all 21 oat chromosomes. A QTL for DON on chromosome 17A/7C, tentatively designated as Qdon.umb-17A/7C, was detected in all experiments using composite interval mapping, with phenotypic effects of 12.2–26.6 %. In addition, QTL for DON were also found on chromosomes 5C, 9D, 13A, 14D and unknown_3, while a QTL for FHB was found on 11A. Several of the DON/FHB QTL coincided with those for DH, DM and/or PH. A half-sib population of HZ595, Hurdal × Z615-4 (HZ615, with 91 lines), was phenotyped in 2011 for validation of QTL found in HZ595, and Qdon.umb-17A/7C was again localized with a phenotypic effect of 12.4 %. Three SNPs closely linked to Qdon.umb-17A/7C were identified in both populations, and one each for QTL on 5C, 11A and 13A were identified in HZ595. These SNPs, together with those yet to be identified, could be useful in marker-assisted selection to pyramiding resistance QTL.

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Acknowledgments

This work was supported by grants from the Norwegian Research Council, Project number 178273, ‘Safe grains: Mycotoxin prevention through resistant wheat and oats’, with co-funding from Graminor Plant Breeding (the breeder of the Hurdal cultivar). The authors are very grateful to Drs. Outi Manninen and Pirjo Tanhuanpää, MTT Agrifood Research Finland, for genotyping the HZ595 population with REMAPs; to Dr. Eric N. Jellen, Brigham Young University, for karyotyping Hurdal and Z595-7; and to Drs. Nicolas A. Tinker and Charlene P. Wight, Agriculture and Agri-Food Canada, for kindly providing the M5 program and technical instructions. In addition, the technical contributions from Qiongxian Lu and Anne Guri Marøy in the lab and Selamawit Tekle and Yalew Tarkegne in the field are highly acknowledged.

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Authors and Affiliations

  1. Department of Plant and Environmental Sciences, Norwegian University of Life Sciences, P.O. Box 5003, 1432, Ås, Norway

    Xinyao He, Helge Skinnes & Åsmund Bjørnstad

  2. USDA-ARS, Small Grains and Potato Germplasm Research Unit, 1691 S. 2700 W, Aberdeen, ID, 83210, USA

    Rebekah E. Oliver & Eric W. Jackson

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  1. Xinyao He
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  2. Helge Skinnes
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  3. Rebekah E. Oliver
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  5. Åsmund Bjørnstad
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Correspondence to Åsmund Bjørnstad.

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Communicated by T. Miedaner.

This paper is dedicated to the memory of Dr. Kenneth J. Frey (March 23, 1923–July 14, 2013), distinguished oat geneticist and breeder through almost 50 years. Two of the parents in this paper, Z595-7 and Z615-4, come from his vast introgression programme from Avena sterilis.

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He, X., Skinnes, H., Oliver, R.E. et al. Linkage mapping and identification of QTL affecting deoxynivalenol (DON) content (Fusarium resistance) in oats (Avena sativa L.). Theor Appl Genet 126, 2655–2670 (2013). https://doi.org/10.1007/s00122-013-2163-0

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