Skip to main content
SpringerLink
Log in

Introgression of stem rust resistance genes SrTA10187 and SrTA10171 from Aegilops tauschii to wheat

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Aegilops tauschii, the diploid progenitor of the wheat D genome, is a readily accessible germplasm pool for wheat breeding as genes can be transferred to elite wheat cultivars through direct hybridization followed by backcrossing. Gene transfer and genetic mapping can be integrated by developing mapping populations during backcrossing. Using direct crossing, two genes for resistance to the African stem rust fungus race TTKSK (Ug99), were transferred from the Ae. tauschii accessions TA10187 and TA10171 to an elite hard winter wheat line, KS05HW14. BC2 mapping populations were created concurrently with developing advanced backcross lines carrying rust resistance. Bulked segregant analysis on the BC2 populations identified marker loci on 6DS and 7DS linked to stem rust resistance genes transferred from TA10187 and TA10171, respectively. Linkage maps were developed for both genes and closely linked markers reported in this study will be useful for selection and pyramiding with other Ug99-effective stem rust resistance genes. The Ae. tauschii-derived resistance genes were temporarily designated SrTA10187 and SrTA10171 and will serve as valuable resources for stem rust resistance breeding.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Anugrahwati DR, Shepherd KW, Verlin DC, Zhang P, Mirzaghaderi G, Walker E, Francki MG, Dundas IS (2008) Isolation of wheat-rye 1RS recombinants that break the linkage between the stem rust resistance gene SrR and secalin. Genome 51:341–349

    Article  PubMed  CAS  Google Scholar 

  • Cox TS (1998) Deepening the wheat gene pool. J Crop Prod 1:1–25

    Article  Google Scholar 

  • Cox TS, Sears RG, Bequette RK (1995a) Use of winter wheat × Triticum tauschii backcross populations for germplasm evaluation. Theor Appl Genet 90:571–577

    Article  Google Scholar 

  • Cox TS, Sears RG, Bequette RK, Martin TJ (1995b) Germplasm enhancement in winter wheat × Triticum tauschii backcross populations. Crop Sci 35:913–919

    Article  Google Scholar 

  • Dundas IS, Anugrahwati DR, Verlin DC, Park RF, Bariana HS, Mago R, Islam AKMR (2007) New sources of rust resistance from alien species: meliorating linked defects and discovery. Aust J Agric Res 58:545–549

    Article  CAS  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158

    Article  PubMed  CAS  Google Scholar 

  • Ghazvini H, Hiebert CW, Zegeye T, Liu S, Dilawari M, Tsilo T, Anderson JA, Rouse MN, Jin Y, Fetch T (2012) Inheritance of resistance to Ug99 stem rust in wheat cultivar Norin 40 and genetic mapping of Sr42. Theor Appl Genet 4:817–824

    Article  Google Scholar 

  • Gill BS, Raupp WJ (1987) Direct genetic transfers from Aegilops squarrosa L. to hexaploid wheat. Crop Sci 27:445–450

    Article  Google Scholar 

  • Hiebert CW, Fetch TG, Zegeye T, Thomas JB, Somers DJ, Humphreys DG, McCallum BD, Cloutier S, Singh D, Knott DR (2011) Genetics and mapping of seedling resistance to Ug99 stem rust in Canadian wheat cultivars ‘Peace’ and ‘AC Cadillac’. Theor Appl Genet 122:143–149

    Article  PubMed  Google Scholar 

  • Jin Y, Singh RP, Ward RW, Wanyera R, Kinyua M, Njau P, Fetch T, Pretorius ZA, Yahyaoui A (2007) Characterization of seedling infection types and adult plant infection responses of monogenic Sr gene lines to race TTKS of Puccinia graminis f. sp. tritici. Plant Dis 91:1096–1099

    Article  Google Scholar 

  • Jin Y, Szabo LJ, Pretorius ZA, Singh RP, Ward R, Fetch T Jr (2008) Detection of virulence to resistance gene Sr24 within race TTKS of Puccinia graminis f. sp. tritici. Plant Dis 92:923–926

    Article  Google Scholar 

  • Kerber ER, Dyck PL (1979) Resistance to stem and leaf rust of wheat in Aegilops squarrosa and transfer of a gene for stem rust resistance to hexaploid wheat. In: Ramanjuam S (ed) Proceedings of the 5th international wheat genetics symposium. New Delhi, India, pp 358–364

    Google Scholar 

  • Klindworth DL, Niu Z, Chao S, Friesen TL, Jin Y, Faris JD, Cai X, Xu SS (2012) Introgression and characterization of a goatgrass gene for a high level of resistance to Ug99 stem rust in tetraploid wheat. G3 Bethesda 2:665–673

    Article  PubMed  CAS  Google Scholar 

  • Kolmer J, Garvin DF, Jin Y (2011) Expression of a Thatcher wheat adult plant stem rust resistance QTL on chromosome arm 2BL is enhanced by Lr34. Crop Sci 51:526–533

    Article  Google Scholar 

  • Krattinger SG, Lagudah ES, Spielmeyer W, Singh RP, Huerta-Espino J, McFadden H, Bossolini E, Selter LL, Keller B (2009) A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science 323:1360–1363

    Article  PubMed  CAS  Google Scholar 

  • Lagudah ES, Krattinger SG, Herrera-Foessel S, Singh RP, Huerta-Espino J, Wolfgang Spielmeyer W, Brown-Guedira G, Selter LL, Keller B (2009) Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens. Theor Appl Genet 119:889–898

    Article  PubMed  CAS  Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newberg LA (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental an natural populations. Genomics 2:174–181

    Article  Google Scholar 

  • Liu W, Jin Y, Rouse M, Friebe B, Gill B, Pumphrey MO (2011a) Development and characterization of wheat-Ae. searsii Robertsonian translocations and a recombinant chromosome conferring resistance to stem rust. Theor Appl Genet 122:1537–1545

    Article  PubMed  Google Scholar 

  • Liu W, Rouse M, Friebe B, Jin Y, Gill B, Pumphrey MO (2011b) Discovery and molecular mapping of a new gene conferring resistance to stem rust, Sr53, derived from Aegilops geniculata and characterization of spontaneous translocation stocks with reduced alien chromatin. Chromosome Res 19:669–682

    Article  PubMed  CAS  Google Scholar 

  • Liu W, Danilova TV, Rouse M, Bowden RL, Friebe B, Gill BS, Pumphrey MO (2013) Development and characterization of a compensating wheat-Thinopyrum intermedium Robertsonian translocation with Sr44 resistance to stem rust (Ug99). Theor Appl Genet. doi:10.1007/s00122-013-2044-6

    Google Scholar 

  • McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts, an atlas of resistance genes. CSIRO, East Melbourne

    Book  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Niu Z, Klindworth DL, Friesen TL, Chao S, Jin Y, Cai X, Xu SS (2011) Targeted introgression of a wheat stem rust resistance gene by DNA marker-assisted chromosome engineering. Genetics 187:1011–1021

    Article  PubMed  CAS  Google Scholar 

  • Olson EL, Brown-Guedira G, Marshall DS, Jin Y, Mergoum M, Lowe I, Dubcovsky J (2010a) Genotyping of US wheat germplasm for presence of stem rust resistance genes Sr24, Sr36 and Sr1RSAmigo. Crop Sci 50:668–675

    Article  CAS  Google Scholar 

  • Olson EL, Brown-Guedira G, Marshall D, Stack E, Bowden RL, Jin Y, Rouse MN, Pumphrey MO (2010b) Development of wheat lines having a small introgressed segment carrying stem rust resistance gene Sr22. Crop Sci 50:1823–1830

    Article  CAS  Google Scholar 

  • Olson EL, Rouse M, Pumphrey MO, Bowden R, Gill BS, Poland JA (2013) Simultaneous transfer, introgression, and genomic localization of genes for resistance to stem rust race TTKSK (Ug99) from Aegilops tauschii to wheat. Theor Appl Genet. doi:10.1007/s00122-013-2045-5

    Google Scholar 

  • Pretorius Z, Singh R, Wagoire W, Payne T (2000) Detection of virulence to wheat stem rust resistance gene Sr31 in Puccinia graminis. f. sp. tritici in Uganda. Plant Dis 84:203

    Article  Google Scholar 

  • Qi LL, Pumphrey MO, Friebe B, Zhang P, Qian C, Bowden RL, Rouse MN, Jin Y, Gill BS (2011) A novel Robertsonian translocation event leads to transfer of a stem rust resistance gene (Sr52) effective against race Ug99 from Dasypyrum villosum into bread wheat. Theor Appl Genet 123:159–167

    Article  PubMed  CAS  Google Scholar 

  • Roelfs AP, Martens JW (1987) An international system of nomenclature for Puccinia graminis f. sp. tritici. Phytopathology 78:526–533

    Article  Google Scholar 

  • Rosenquist CE (1927) An improved method of producing F1 hybrid seeds of wheat and barley. Agron J 19:968–971

    Article  Google Scholar 

  • Rouse MN, Olson EL, Gill BS, Pumphrey MO, Jin Y (2011) Stem rust resistance in Aegilops tauschii germplasm. Crop Sci 51:2074–2078

    Article  Google Scholar 

  • Rubiales D, Niks RE (1995) Characterisation of Lr34, a major gene conferring non-hypersensitive resistance to wheat leaf rust. Plant Dis 79:1208–1212

    Article  Google Scholar 

  • Sears ER (1948) The cytology and genetics of the wheats and their relatives. Adv Genet 2:239–270

    Article  Google Scholar 

  • Singh RP, Hodson DP, Huerta-Espino J, Jin Y, Bhavani S, Njau P, Herrera-Foessel S, Singh PK, Singh S, Govindan V (2011) The emergence of Ug99 races of the stem rust fungus is a threat to world wheat production. Annu Rev Phytopathol 49:465–481

    Article  PubMed  CAS  Google Scholar 

  • Spielmeyer W, McIntosh RA, Kolmer J, Lagudah ES (2005) Powdery mildew resistance and Lr34/Yr18 genes for durable resistance to leaf and stripe rust cosegregate at a locus on the short arm of chromosome 7D of wheat. Theor Appl Genet 111:731–735

    Article  PubMed  CAS  Google Scholar 

  • Stakman E, Steward D, Loegering W (1962) Identification of physiologic races of Puccinia graminis var. tritici. US Department of Agriculture, ARS, Washington, p E-617

    Google Scholar 

Download references

Acknowledgments

This is contribution number 13-105-J from the Kansas Agricultural Experiment Station. This work was funded by the Durable Rust Resistance in Wheat project, Cornell University through a grant from The Bill & Melinda Gates Foundation and the USDA-ARS (Appropriation #5430-21000-006-00D). We thank Amy Bernardo, Paul St. Amand, Katherine Kaus, and Mitchell Keller for technical assistance. Dr. Robert A. McIntosh gave suggestions that improved this manuscript.

Author information

Author notes

  1. Eric L. Olson

    Present address: Department of Plant and Soil Science, Michigan State University, East Lansing, MI, 48824, USA

Authors and Affiliations

  1. Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506, USA

    Eric L. Olson, Robert L. Bowden & Bikram S. Gill

  2. USDA-ARS Cereal Disease Laboratory, University of Minnesota, St. Paul, MN, 55108, USA

    Matthew N. Rouse

  3. Department of Crop and Soil Sciences, Washington State University, Pullman, WA, 99164-6420, USA

    Michael O. Pumphrey

  4. Hard Winter Wheat Genetics Research Unit, USDA-ARS, Manhattan, KS, 66502, USA

    Robert L. Bowden & Jesse A. Poland

  5. Department of Agronomy, Kansas State University, Manhattan, KS, 66506, USA

    Jesse A. Poland

Authors
  1. Eric L. Olson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Matthew N. Rouse
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Michael O. Pumphrey
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Robert L. Bowden
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Bikram S. Gill
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jesse A. Poland
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jesse A. Poland.

Additional information

Communicated by S. Dreisigacker.

Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Olson, E.L., Rouse, M.N., Pumphrey, M.O. et al. Introgression of stem rust resistance genes SrTA10187 and SrTA10171 from Aegilops tauschii to wheat. Theor Appl Genet 126, 2477–2484 (2013). https://doi.org/10.1007/s00122-013-2148-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-013-2148-z

Keywords

Search

Navigation