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FR-H3: a new QTL to assist in the development of fall-sown barley with superior low temperature tolerance

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Abstract

Fall-sown barley will be increasingly important in the era of climate change due to higher yield potential and efficient use of water resources. Resistance/tolerance to abiotic stresses will be critical, and foremost among the abiotic stresses is low temperature. Simultaneous gene discovery and breeding will accelerate the development of agronomically relevant fall-sown barley germplasm with resistance to low temperature. We developed two doubled haploid mapping populations using two lines from the University of Nebraska (NE) and one line from Oregon State University (OR): NB3437f/OR71 (facultative × facultative) and NB713/OR71 (winter × facultative). Both were genotyped with a custom 384 oligonucleotide pool assay (OPA). QTL analyses were performed for low temperature tolerance (LTT) and vernalization sensitivity (VS). The role of VRN-H2 in VS was confirmed and a novel alternative winter allele at VRN-H3 was discovered in the Nebraska germplasm. FR-H2 was identified as a probable determinant of LTT and a new QTL, FR-H3, was discovered on chromosome 1H that accounted for up to 48 % of the phenotypic variation in field survival at St. Paul, MN, USA. The discovery of FR-H3 is a significant advancement in barley LTT genetics and will assist in developing the next generation of fall-sown varieties.

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Acknowledgments

We would like to thank Blake Cooper (Limagrain USA) and Chris Evans (Busch Agricultural Resources, Inc., USA) for the field phenotyping of LTT at Fort Collins, Colorado and Fairfield, Montana (USA). This work was supported by the Agriculture and Food Research Initiative Plant Genome, Genetics and Breeding Program of USDA’s National Institute of Food and Agriculture (2009-65300-05666).

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Authors and Affiliations

  1. Department of Crop and Soil Science, Oregon State University, Corvallis, USA

    Scott P. Fisk, Alfonso Cuesta-Marcos, Ann Corey, Tanya Filichkin & Patrick M. Hayes

  2. Genetica y produccion Vegetal, Estacion Experimental de Aula Dei, Consejo Superior de Investigaciones Cientificas, Zaragoza, Spain

    Luis Cistué

  3. Department of Cell and Molecular Sciences, The James Hutton Institute, Invergowrie, Dundee, Scotland, UK

    Joanne Russell & Robbie Waugh

  4. Agronomy and Plant Genetics, University of Minnesota, Saint Paul, USA

    Kevin P. Smith

  5. Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, USA

    Stephen Baenziger

  6. Agricultural Research Institute of the Hungarian Academy of Sciences, Martonvásár, Hungary

    Zoltán Bedo & Ildikó Karsai

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  1. Scott P. Fisk
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  2. Alfonso Cuesta-Marcos
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  3. Luis Cistué
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  7. Zoltán Bedo
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  8. Ann Corey
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  10. Ildikó Karsai
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  11. Robbie Waugh
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  12. Patrick M. Hayes
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Correspondence to Alfonso Cuesta-Marcos.

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Communicated by A. Graner.

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Fisk, S.P., Cuesta-Marcos, A., Cistué, L. et al. FR-H3: a new QTL to assist in the development of fall-sown barley with superior low temperature tolerance. Theor Appl Genet 126, 335–347 (2013). https://doi.org/10.1007/s00122-012-1982-8

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  • DOI: https://doi.org/10.1007/s00122-012-1982-8

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