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Identification of Indica rice chromosome segments for the improvement of Japonica inbreds and hybrids

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Abstract

Exploitation of heterosis has brought significant advance in plant breeding and agricultural production, although its genetic basis is still poorly understood. In this study, a total of 66 chromosome segment substitution (CSS) lines, derived from a cross between japonica rice inbred line Asominori (as the recurrent parent) and indica rice inbred line IR24 (as the donor parent), were used to investigate the genetic basis of heterosis in indica × japonica inter-subspecific rice hybrids. Each CSS line was crossed with the background parent Asominori, and the heterosis of F1 hybrids was estimated by comparing the F1 performance with its two parental lines. Field experiments were carried out across six different environments to evaluate yield and yield-related traits in the 66 CSS lines and their 66 corresponding F1 hybrids. Quantitative trait loci (QTL) analyses were conducted using a likelihood ratio test based on the stepwise regression. Thirty-six QTL were identified with significant effects in CSSL, 21 with significant effects in hybrids and 13 with significant effects in both. On the basis of average dominance degree, of all the 70 QTL affecting yield-related agronomic traits, 28.6% (20) showed an overdominance, 35.7% (25) a partial dominance and 30% (21) an additive effect, indicating that all effects contribute to trait variation in japonica–indica rice hybrids. Effects of these QTL were examined to identify Indica rice chromosome segments of interest for the improvement of japonica inbred lines and hybrids.

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Acknowledgments

We thank the editor and two anonymous reviewers for their constructive comments which significantly improved the manuscript. We also thank Professor A. Yoshimura, Kyushu University, Japan, for kindly providing us with the CSS lines population. This research is supported by the grants from the 863 Program of China (2009AA101101), the 973 Program of China (2011CB100102), the earmarked fund for Modern Agro-industry Technology Research System, Jiangsu Science and Technology Development Program (BE2009301-3), and PAPD project.

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Authors and Affiliations

  1. State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Provincial Center of Plant Gene Engineering, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, China

    Zhiquan Wang, Chuanyuan Yu, Xi Liu, Shijia Liu, Linglong Liu, Ling Jiang, Chao Yang, Liangming Chen & Jianmin Wan

  2. Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang, 330200, China

    Zhiquan Wang, Chuanyuan Yu & Jianguo Lei

  3. Institute of Crop Science, The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, 10081, China

    Changbin Yin, Huqu Zhai & Jianmin Wan

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  1. Zhiquan Wang
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  2. Chuanyuan Yu
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  3. Xi Liu
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  4. Shijia Liu
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  8. Ling Jiang
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  10. Liangming Chen
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  12. Jianmin Wan
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Correspondence to Jianmin Wan.

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Communicated by A. Charcosset.

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Wang, Z., Yu, C., Liu, X. et al. Identification of Indica rice chromosome segments for the improvement of Japonica inbreds and hybrids. Theor Appl Genet 124, 1351–1364 (2012). https://doi.org/10.1007/s00122-012-1792-z

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  • DOI: https://doi.org/10.1007/s00122-012-1792-z

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