Abstract
Asian cultivated rice (Oryza sativa L.) has two subspecies, indica and japonica, which display clear differences in yield-related traits and environmental adaptation. Here, we developed a set of chromosome segment substitution lines (CSSLs) from an advanced backcross between japonica variety C418, as the recipient, and indica variety IR24, as the donor. Through evaluating the genotypes and phenotypes of 181 CSSLs, a total of 85 quantitative trait loci (QTLs) for 14 yield-related traits were detected, with individual QTLs explaining from 6.2 to 42.9% of the phenotypic variation. Moreover, twenty-six of these QTLs could be detected in the two trial sites (Beijing and Hainan). Among these loci, the QTLs for flag leaf width and effective tiller number, qFLW4.2 and qETN4.2, were delimited to an approximately 256-kb interval on chromosome 4. Through a comparison of nucleotide sequences and expression levels in “C418” and the CSSL CR31 containing qFLW4.2 and qETN4.2, we found that the NAL1 (LOC_Os04g52479) gene was the candidate gene for qFLW4.2 and qETN4.2. Our results show that CSSLs are powerful tools for identifying and fine-mapping QTLs, while the novel QTLs identified in this study will also provide new genetic resources for rice improvement.
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This work was supported by the National Nature Science Foundation of China (32125029) and the self-regulated project of the State Key Laboratory of Agrobiotechnology (2022SKLAB1-3).
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LT conceived and designed the experiments; SL performed most of the experiments; JZ, JF, and DG provided technical assistance and developed the CSSL population; LT and SL performed data analysis and wrote the article.
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Li, S., Zou, J., Fan, J. et al. Identification of quantitative trait loci for important agronomic traits using chromosome segment substitution lines from a japonica × indica cross in rice. Mol Breeding 42, 73 (2022). https://doi.org/10.1007/s11032-022-01343-3
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DOI: https://doi.org/10.1007/s11032-022-01343-3